A+ U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein d gene expression is regulated by oestrogen in the rat uterus

Yukitomo Arao, Atsumi Kikuchi, Kazuhiro Ikeda, Satoshi Nomoto, Hyogo Horiguchi, Fujio Kayama

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Oestrogen-mediated gene expression is regulated at both the transcriptional and post-transcriptional levels. The molecular mechanism of transcriptional regulation has been well characterized. On the other hand, there is little understanding of the mechanism of post-transcriptional regulation. To clarify the mechanism of oestrogen-mediated post-transcriptional regulation, we focused on A+U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein D (AUF1/hnRNP D), which is known as a regulator of cytosolic mRNA degradation and nuclear pre-mRNA maturation. However, little is known about the expression levels and the regulation of AUF1/hnRNP D mRNA in tissues. We further investigated the expression levels of AUF1/hnRNP D isoform mRNAs to determine whether AUF1/hnRNP D gene expression is regulated by oestrogen in the ovariectomized adult female rat uterus. Uterine AUF1/hnRNP D mRNA was induced by a single subcutaneous injection (1 μg/kg) of 17β-oestradiol (E2), reaching a peak level within 6 h. Furthermore, we observed that the E2-induced AUF1/hnRNP D isoform mRNAs are p45 and p40 transcripts, and that E2-mediated induction is suppressed by the oestrogen receptor antagonist ICI 182,780. Finally, using the transcriptional inhibitor actinomycin D, we confirmed that the E2-mediated increase in AUF1/hnRNP D mRNA is caused by E2-dependent AUF1/hnRNP D mRNA stabilization.

Original languageEnglish
Pages (from-to)125-132
Number of pages8
JournalBiochemical Journal
Volume361
Issue number1
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Heterogeneous-Nuclear Ribonucleoprotein D
Heterogeneous-Nuclear Ribonucleoproteins
Gene expression
Uterus
Rats
Estrogens
RNA
Gene Expression
Messenger RNA
RNA Isoforms
RNA Precursors
RNA Stability
Dactinomycin
Subcutaneous Injections
Estradiol
Stabilization

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A+ U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein d gene expression is regulated by oestrogen in the rat uterus. / Arao, Yukitomo; Kikuchi, Atsumi; Ikeda, Kazuhiro; Nomoto, Satoshi; Horiguchi, Hyogo; Kayama, Fujio.

In: Biochemical Journal, Vol. 361, No. 1, 01.01.2002, p. 125-132.

Research output: Contribution to journalArticle

Arao, Yukitomo ; Kikuchi, Atsumi ; Ikeda, Kazuhiro ; Nomoto, Satoshi ; Horiguchi, Hyogo ; Kayama, Fujio. / A+ U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein d gene expression is regulated by oestrogen in the rat uterus. In: Biochemical Journal. 2002 ; Vol. 361, No. 1. pp. 125-132.
@article{30a4c5a736844523af56e4cf1411aca0,
title = "A+ U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein d gene expression is regulated by oestrogen in the rat uterus",
abstract = "Oestrogen-mediated gene expression is regulated at both the transcriptional and post-transcriptional levels. The molecular mechanism of transcriptional regulation has been well characterized. On the other hand, there is little understanding of the mechanism of post-transcriptional regulation. To clarify the mechanism of oestrogen-mediated post-transcriptional regulation, we focused on A+U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein D (AUF1/hnRNP D), which is known as a regulator of cytosolic mRNA degradation and nuclear pre-mRNA maturation. However, little is known about the expression levels and the regulation of AUF1/hnRNP D mRNA in tissues. We further investigated the expression levels of AUF1/hnRNP D isoform mRNAs to determine whether AUF1/hnRNP D gene expression is regulated by oestrogen in the ovariectomized adult female rat uterus. Uterine AUF1/hnRNP D mRNA was induced by a single subcutaneous injection (1 μg/kg) of 17β-oestradiol (E2), reaching a peak level within 6 h. Furthermore, we observed that the E2-induced AUF1/hnRNP D isoform mRNAs are p45 and p40 transcripts, and that E2-mediated induction is suppressed by the oestrogen receptor antagonist ICI 182,780. Finally, using the transcriptional inhibitor actinomycin D, we confirmed that the E2-mediated increase in AUF1/hnRNP D mRNA is caused by E2-dependent AUF1/hnRNP D mRNA stabilization.",
author = "Yukitomo Arao and Atsumi Kikuchi and Kazuhiro Ikeda and Satoshi Nomoto and Hyogo Horiguchi and Fujio Kayama",
year = "2002",
month = "1",
day = "1",
doi = "10.1042/0264-6021:3610125",
language = "English",
volume = "361",
pages = "125--132",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "1",

}

TY - JOUR

T1 - A+ U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein d gene expression is regulated by oestrogen in the rat uterus

AU - Arao, Yukitomo

AU - Kikuchi, Atsumi

AU - Ikeda, Kazuhiro

AU - Nomoto, Satoshi

AU - Horiguchi, Hyogo

AU - Kayama, Fujio

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Oestrogen-mediated gene expression is regulated at both the transcriptional and post-transcriptional levels. The molecular mechanism of transcriptional regulation has been well characterized. On the other hand, there is little understanding of the mechanism of post-transcriptional regulation. To clarify the mechanism of oestrogen-mediated post-transcriptional regulation, we focused on A+U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein D (AUF1/hnRNP D), which is known as a regulator of cytosolic mRNA degradation and nuclear pre-mRNA maturation. However, little is known about the expression levels and the regulation of AUF1/hnRNP D mRNA in tissues. We further investigated the expression levels of AUF1/hnRNP D isoform mRNAs to determine whether AUF1/hnRNP D gene expression is regulated by oestrogen in the ovariectomized adult female rat uterus. Uterine AUF1/hnRNP D mRNA was induced by a single subcutaneous injection (1 μg/kg) of 17β-oestradiol (E2), reaching a peak level within 6 h. Furthermore, we observed that the E2-induced AUF1/hnRNP D isoform mRNAs are p45 and p40 transcripts, and that E2-mediated induction is suppressed by the oestrogen receptor antagonist ICI 182,780. Finally, using the transcriptional inhibitor actinomycin D, we confirmed that the E2-mediated increase in AUF1/hnRNP D mRNA is caused by E2-dependent AUF1/hnRNP D mRNA stabilization.

AB - Oestrogen-mediated gene expression is regulated at both the transcriptional and post-transcriptional levels. The molecular mechanism of transcriptional regulation has been well characterized. On the other hand, there is little understanding of the mechanism of post-transcriptional regulation. To clarify the mechanism of oestrogen-mediated post-transcriptional regulation, we focused on A+U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein D (AUF1/hnRNP D), which is known as a regulator of cytosolic mRNA degradation and nuclear pre-mRNA maturation. However, little is known about the expression levels and the regulation of AUF1/hnRNP D mRNA in tissues. We further investigated the expression levels of AUF1/hnRNP D isoform mRNAs to determine whether AUF1/hnRNP D gene expression is regulated by oestrogen in the ovariectomized adult female rat uterus. Uterine AUF1/hnRNP D mRNA was induced by a single subcutaneous injection (1 μg/kg) of 17β-oestradiol (E2), reaching a peak level within 6 h. Furthermore, we observed that the E2-induced AUF1/hnRNP D isoform mRNAs are p45 and p40 transcripts, and that E2-mediated induction is suppressed by the oestrogen receptor antagonist ICI 182,780. Finally, using the transcriptional inhibitor actinomycin D, we confirmed that the E2-mediated increase in AUF1/hnRNP D mRNA is caused by E2-dependent AUF1/hnRNP D mRNA stabilization.

UR - http://www.scopus.com/inward/record.url?scp=0036179339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036179339&partnerID=8YFLogxK

U2 - 10.1042/0264-6021:3610125

DO - 10.1042/0264-6021:3610125

M3 - Article

C2 - 11742537

AN - SCOPUS:0036179339

VL - 361

SP - 125

EP - 132

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -