A unique and common restriction fragment pattern of the nucleotide sequences homologous to the genome of Vf33, a filamentous bacteriophage, in pandemic strains of Vibrio parahaemolyticus O3:K6 O4:K68, and O1:K untypeable

Bin Chang, Shin Ichi Yoshida, Hiroshi Miyamoto, Midori Ogawa, Kazumi Horikawa, Kikuyo Ogata, Mitsuaki Nishibuchi, Hatsumi Taniguchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1999) of V. parahaemolyticus. Nucleotide sequences homologous to the Vf33 DNA were detected in all 57 test strains including pandemic and non-pandemic strains. However, the profiles of hybridization, including the restriction fragment length polymorphism, with nine Vf33-derived DNA probes exhibited by the pandemic strains were identical and were different from those by the non-pandemic strains. The results support the hypothesis that the pandemic strains are clonal, and suggest a possibility that they have acquired (a) new gene(s) via a Vf33-like filamentous phage. (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalFEMS microbiology letters
Volume192
Issue number2
DOIs
Publication statusPublished - Nov 15 2000
Externally publishedYes

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Inovirus
Vibrio parahaemolyticus
Pandemics
Genome
DNA Probes
Southern Blotting
Restriction Fragment Length Polymorphisms
Bacteriophages
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

A unique and common restriction fragment pattern of the nucleotide sequences homologous to the genome of Vf33, a filamentous bacteriophage, in pandemic strains of Vibrio parahaemolyticus O3:K6 O4:K68, and O1:K untypeable. / Chang, Bin; Yoshida, Shin Ichi; Miyamoto, Hiroshi; Ogawa, Midori; Horikawa, Kazumi; Ogata, Kikuyo; Nishibuchi, Mitsuaki; Taniguchi, Hatsumi.

In: FEMS microbiology letters, Vol. 192, No. 2, 15.11.2000, p. 231-236.

Research output: Contribution to journalArticle

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abstract = "Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1999) of V. parahaemolyticus. Nucleotide sequences homologous to the Vf33 DNA were detected in all 57 test strains including pandemic and non-pandemic strains. However, the profiles of hybridization, including the restriction fragment length polymorphism, with nine Vf33-derived DNA probes exhibited by the pandemic strains were identical and were different from those by the non-pandemic strains. The results support the hypothesis that the pandemic strains are clonal, and suggest a possibility that they have acquired (a) new gene(s) via a Vf33-like filamentous phage. (C) 2000 Federation of European Microbiological Societies.",
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AU - Chang, Bin

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