A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors

Nathalie Brouard, Camille Jost, Nadine Matthias, Camille Albrecht, Sébastien Egard, Poojabahen Gandhi, Catherine Strassel, Tomoko Inoue, Daisuke Sugiyama, Paul J. Simmons, Christian Gachet, Francois Lanza

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The fetal liver is the site of a major expansion of the hematopoietic stem cell (HSC) pool and is also a privileged organ to study megakaryocyte progenitor differentiation. We identified in the mouse fetal liver at day 13.5 a discrete stromal cell population harboring a CD45-TER119-CD31-CD51+VCAM-1+PDGFRα- (V+P-) phenotype that lacked colonyforming unit fibroblast activity and harbored an hepatocyte progenitor signature. This previously undescribed V1P2 population efficiently supported megakaryocyte production from mouse bone marrow HSC and human peripheral blood HSC-myeloid progenitors cultured in the presence of limited cytokine concentrations. Megakaryocytes obtained in V1P2 cocultures were polyploid, positive for CD41/CD42c, and efficiently produced proplatelets. Megakaryocyte production appeared to be mediated by an expansion of the progenitor compartment through HSC-stromal cell contact. In conclusion, the fetal liver contains a unique cellular microenvironment that could represent a platform for the discovery of regulators of megakaryopoiesis.

Original languageEnglish
Pages (from-to)1854-1866
Number of pages13
JournalBlood Advances
Volume1
Issue number21
DOIs
Publication statusPublished - Sep 26 2017

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Megakaryocyte Progenitor Cells
Hematopoietic Stem Cells
Megakaryocytes
Liver
Stromal Cells
Cellular Microenvironment
Polyploidy
Vascular Cell Adhesion Molecule-1
Coculture Techniques
Population
Hepatocytes
Fibroblasts
Bone Marrow
Cytokines
Phenotype

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Brouard, N., Jost, C., Matthias, N., Albrecht, C., Egard, S., Gandhi, P., ... Lanza, F. (2017). A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors. Blood Advances, 1(21), 1854-1866. https://doi.org/10.1182/bloodadvances.2016003541

A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors. / Brouard, Nathalie; Jost, Camille; Matthias, Nadine; Albrecht, Camille; Egard, Sébastien; Gandhi, Poojabahen; Strassel, Catherine; Inoue, Tomoko; Sugiyama, Daisuke; Simmons, Paul J.; Gachet, Christian; Lanza, Francois.

In: Blood Advances, Vol. 1, No. 21, 26.09.2017, p. 1854-1866.

Research output: Contribution to journalArticle

Brouard, N, Jost, C, Matthias, N, Albrecht, C, Egard, S, Gandhi, P, Strassel, C, Inoue, T, Sugiyama, D, Simmons, PJ, Gachet, C & Lanza, F 2017, 'A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors', Blood Advances, vol. 1, no. 21, pp. 1854-1866. https://doi.org/10.1182/bloodadvances.2016003541
Brouard, Nathalie ; Jost, Camille ; Matthias, Nadine ; Albrecht, Camille ; Egard, Sébastien ; Gandhi, Poojabahen ; Strassel, Catherine ; Inoue, Tomoko ; Sugiyama, Daisuke ; Simmons, Paul J. ; Gachet, Christian ; Lanza, Francois. / A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors. In: Blood Advances. 2017 ; Vol. 1, No. 21. pp. 1854-1866.
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