A YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines

Kiyoyuki Torigoe, Seiji Sato, Hitoshi Kusaba, Kimitoshi Kohno, Michihiko Kuwano, Katsuzumi Okumura, Eric D. Green, Lap Chee Tsui, Stephen W. Scherer, David Schlessinger, Morimasa Wada

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

A contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.

Original languageEnglish
Pages (from-to)233-244
Number of pages12
JournalGenome Research
Volume5
Issue number3
DOIs
Publication statusPublished - Oct 1995

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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