A yeast one-hybrid system to screen for methylated DNA-binding proteins

Shu Ying Feng, Kazuhisa Ota, Takashi Ito

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We had previously exploited a method for targeted DNA methylation in budding yeast to succeed in one-hybrid detection of methylation-dependent DNA-protein interactions. Based on this finding, we developed a yeast one-hybrid system to screen cDNA libraries for clones encoding methylated DNA-binding proteins. Concurrent use of two independent bait sequences in the same cell, or dual-bait system, effectively reduced false positive clones, which were derived from methylationinsensitive sequence-specific DNA-binding proteins. We applied the dual-bait system to screen cDNA libraries and demonstrated efficient isolation of clones for methylated DNA-binding proteins. This system would serve as a unique research tool for epigenetics.

Original languageEnglish
Pages (from-to)e189
JournalNucleic acids research
Volume38
Issue number20
DOIs
Publication statusPublished - Nov 1 2010
Externally publishedYes

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DNA-Binding Proteins
Clone Cells
Yeasts
DNA Methylation
Gene Library
Saccharomycetales
Epigenomics
Research
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

A yeast one-hybrid system to screen for methylated DNA-binding proteins. / Feng, Shu Ying; Ota, Kazuhisa; Ito, Takashi.

In: Nucleic acids research, Vol. 38, No. 20, 01.11.2010, p. e189.

Research output: Contribution to journalArticle

Feng, Shu Ying ; Ota, Kazuhisa ; Ito, Takashi. / A yeast one-hybrid system to screen for methylated DNA-binding proteins. In: Nucleic acids research. 2010 ; Vol. 38, No. 20. pp. e189.
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