A yeast one-hybrid system to screen for methylated DNA-binding proteins

Shu Ying Feng, Kazuhisa Ota, Takashi Ito

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We had previously exploited a method for targeted DNA methylation in budding yeast to succeed in one-hybrid detection of methylation-dependent DNA-protein interactions. Based on this finding, we developed a yeast one-hybrid system to screen cDNA libraries for clones encoding methylated DNA-binding proteins. Concurrent use of two independent bait sequences in the same cell, or dual-bait system, effectively reduced false positive clones, which were derived from methylationinsensitive sequence-specific DNA-binding proteins. We applied the dual-bait system to screen cDNA libraries and demonstrated efficient isolation of clones for methylated DNA-binding proteins. This system would serve as a unique research tool for epigenetics.

Original languageEnglish
Pages (from-to)e189
JournalNucleic acids research
Volume38
Issue number20
DOIs
Publication statusPublished - Nov 2010

All Science Journal Classification (ASJC) codes

  • Genetics

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