Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients

Hisato Kobayashi, Akiko Sato, Eiko Otsu, Hitoshi Hiura, Chisako Tomatsu, Takafumi Utsunomiya, Hiroyuki Sasaki, Nobuo Yaegashi, Takahiro Arima

Research output: Contribution to journalArticle

278 Citations (Scopus)

Abstract

Recent studies suggest that assisted reproductive technologies (ART), which involve the isolation, handling and culture of gametes and early embryos, are associated with an increased incidence of rare imprinting disorders. Major epigenetic events take place during this time and the process of ART may expose the epigenome to external influences, preventing the proper establishment and maintenance of genomic imprints. However, the risks of ART cannot be simply evaluated because the patients who receive ART may differ both demographically and genetically from the general population at reproductive age. In this study, we examined the DNA methylation status of seven imprinted genes using a combined bisulphite-PCR restriction analysis and sequencing technique on sperm DNA obtained from 97 infertile men. We found an abnormal paternal methylation imprint in 14 patients (14.4%) and abnormal maternal imprint in 20 patients (20.6%). The majority of these doubly defective samples were in men with moderate or severe oligospermia. These abnormalities were specific to imprinted loci as we found that global DNA methylation was normal in these samples. The outcome of ART with sperm shown to have an abnormal DNA methylation pattern was generally poor. However, one sample of sperm with both paternal and maternal methylation errors used in ICSI produced a child of normal appearance without any abnormalities in their imprinted methylation pattern. Our data suggest that sperm from infertile patients, especially those with oligospermia, may carry a higher risk of transmitting incorrect primary imprints to their offspring, highlighting the need for more research into ART.

Original languageEnglish
Pages (from-to)2542-2551
Number of pages10
JournalHuman Molecular Genetics
Volume16
Issue number21
DOIs
Publication statusPublished - Nov 1 2007

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Assisted Reproductive Techniques
DNA Methylation
Spermatozoa
Methylation
Oligospermia
Mothers
Intracytoplasmic Sperm Injections
Epigenomics
Germ Cells
Embryonic Structures
Maintenance
Polymerase Chain Reaction
DNA
Incidence
Research
Population
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Kobayashi, H., Sato, A., Otsu, E., Hiura, H., Tomatsu, C., Utsunomiya, T., ... Arima, T. (2007). Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Human Molecular Genetics, 16(21), 2542-2551. https://doi.org/10.1093/hmg/ddm187

Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. / Kobayashi, Hisato; Sato, Akiko; Otsu, Eiko; Hiura, Hitoshi; Tomatsu, Chisako; Utsunomiya, Takafumi; Sasaki, Hiroyuki; Yaegashi, Nobuo; Arima, Takahiro.

In: Human Molecular Genetics, Vol. 16, No. 21, 01.11.2007, p. 2542-2551.

Research output: Contribution to journalArticle

Kobayashi, H, Sato, A, Otsu, E, Hiura, H, Tomatsu, C, Utsunomiya, T, Sasaki, H, Yaegashi, N & Arima, T 2007, 'Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients', Human Molecular Genetics, vol. 16, no. 21, pp. 2542-2551. https://doi.org/10.1093/hmg/ddm187
Kobayashi H, Sato A, Otsu E, Hiura H, Tomatsu C, Utsunomiya T et al. Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Human Molecular Genetics. 2007 Nov 1;16(21):2542-2551. https://doi.org/10.1093/hmg/ddm187
Kobayashi, Hisato ; Sato, Akiko ; Otsu, Eiko ; Hiura, Hitoshi ; Tomatsu, Chisako ; Utsunomiya, Takafumi ; Sasaki, Hiroyuki ; Yaegashi, Nobuo ; Arima, Takahiro. / Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. In: Human Molecular Genetics. 2007 ; Vol. 16, No. 21. pp. 2542-2551.
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