Aberrant HS1 molecule in a patient with systemic lupus erythematosus

T. Sawabe, T. Horiuchi, R. Koga, H. Tsukamoto, T. Kojima, T. Harashima, Y. Kikuchi, J. Otsuka, H. Mitoma, S. Yoshizawa, Y. Niho, T. Watanabe

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the activation of autoreactive B lymphocytes, which are supposed to carry aberrant signal transduction after the stimulation of B-cell receptor (BCR). To investigate abnormalities in BCR-mediated signaling pathway in lupus B lymphocytes, we analyzed HS1, a molecule downstream of BCR, in 80 Japanese SLE patients. We identified 37 amino acid deletion of HS1 in a 25-year-old female patient, and the aberrant HS1 lacked a part of a functional motif. Analysis of genomic DNA revealed that the aberrant HS1 was caused by exon skipping. Family study showed that the patient as well as her father and sister are heterozygous for the abnormality. WEHI-231 cell, a mouse B cell line, transfected with the aberrant HS1 displayed a significantly increased cell death upon cross-linking of BCR. Additionally, peripheral B lymphocytes from the patient exerted increased apoptosis after BCR stimulation compared to those from control SLE patients. These data suggest that the aberrant HS1 molecule may transmit an accelerated signal after BCR stimulation and may play a role in the activation of autoreactive B lymphocytes.

Original languageEnglish
Pages (from-to)122-131
Number of pages10
JournalGenes and Immunity
Volume4
Issue number2
DOIs
Publication statusPublished - Mar 1 2003

Fingerprint

Systemic Lupus Erythematosus
B-Lymphocytes
Fathers
Autoimmune Diseases
Siblings
Exons
Signal Transduction
Cell Death
Apoptosis
Amino Acids
Cell Line
DNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics
  • Genetics(clinical)

Cite this

Sawabe, T., Horiuchi, T., Koga, R., Tsukamoto, H., Kojima, T., Harashima, T., ... Watanabe, T. (2003). Aberrant HS1 molecule in a patient with systemic lupus erythematosus. Genes and Immunity, 4(2), 122-131. https://doi.org/10.1038/sj.gene.6363932

Aberrant HS1 molecule in a patient with systemic lupus erythematosus. / Sawabe, T.; Horiuchi, T.; Koga, R.; Tsukamoto, H.; Kojima, T.; Harashima, T.; Kikuchi, Y.; Otsuka, J.; Mitoma, H.; Yoshizawa, S.; Niho, Y.; Watanabe, T.

In: Genes and Immunity, Vol. 4, No. 2, 01.03.2003, p. 122-131.

Research output: Contribution to journalReview article

Sawabe, T, Horiuchi, T, Koga, R, Tsukamoto, H, Kojima, T, Harashima, T, Kikuchi, Y, Otsuka, J, Mitoma, H, Yoshizawa, S, Niho, Y & Watanabe, T 2003, 'Aberrant HS1 molecule in a patient with systemic lupus erythematosus', Genes and Immunity, vol. 4, no. 2, pp. 122-131. https://doi.org/10.1038/sj.gene.6363932
Sawabe, T. ; Horiuchi, T. ; Koga, R. ; Tsukamoto, H. ; Kojima, T. ; Harashima, T. ; Kikuchi, Y. ; Otsuka, J. ; Mitoma, H. ; Yoshizawa, S. ; Niho, Y. ; Watanabe, T. / Aberrant HS1 molecule in a patient with systemic lupus erythematosus. In: Genes and Immunity. 2003 ; Vol. 4, No. 2. pp. 122-131.
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