Aberrant structures of fecal bacterial community in allergic infants profiled by 16S rRNA gene pyrosequencing

Jiro Nakayama, Takako Kobayashi, Shigemitsu Tanaka, Yuki Korenori, Atsushi Tateyama, Naoshige Sakamoto, Chikako Kiyohara, Taro Shirakawa, Kenji Sonomoto

Research output: Contribution to journalArticle

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Abstract

We investigated the correlation between fecal bacteria composition in early infancy and the prevalence of allergic diseases in late infancy. The fecal microbiota in the first 2 months was profiled using the 16S rRNA V6 short-tag sequences in the community and statistically compared between two groups of subjects who did and did not show allergic symptoms in the first 2 years (n = 11 vs. 11). In the allergic group, genus Bacteroides at 1 month and genera Propionibacterium and Klebsiella at 2 months were more abundant, and genera Acinetobacter and Clostridium at 1 month were less abundant than in the nonallergic group. Allergic infants who showed high colonization of Bacteroides and/or Klebsiella showed less colonization of Clostridium perfringens/butyricum, suggesting antagonism between these bacterial groups in the gastrointestinal tract. It was also remarkable that the relative abundance of total Proteobacteria, excluding genus Klebsiella, was significantly lower in the allergic than in the nonallergic group at the age of 1 month. These results indicate that pyrosequence-based 16S rRNA gene profiling is valid to find the intestinal microbiotal disorder that correlates with allergy development in later life.

Original languageEnglish
Pages (from-to)397-406
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume63
Issue number3
DOIs
Publication statusPublished - Dec 1 2011

Fingerprint

Bacterial Structures
Klebsiella
rRNA Genes
Bacteroides
Clostridium butyricum
Propionibacterium
Proteobacteria
Clostridium perfringens
Acinetobacter
Clostridium
Microbiota
Gastrointestinal Tract
Hypersensitivity
Age Groups
Bacteria

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Aberrant structures of fecal bacterial community in allergic infants profiled by 16S rRNA gene pyrosequencing. / Nakayama, Jiro; Kobayashi, Takako; Tanaka, Shigemitsu; Korenori, Yuki; Tateyama, Atsushi; Sakamoto, Naoshige; Kiyohara, Chikako; Shirakawa, Taro; Sonomoto, Kenji.

In: FEMS Immunology and Medical Microbiology, Vol. 63, No. 3, 01.12.2011, p. 397-406.

Research output: Contribution to journalArticle

Nakayama, Jiro ; Kobayashi, Takako ; Tanaka, Shigemitsu ; Korenori, Yuki ; Tateyama, Atsushi ; Sakamoto, Naoshige ; Kiyohara, Chikako ; Shirakawa, Taro ; Sonomoto, Kenji. / Aberrant structures of fecal bacterial community in allergic infants profiled by 16S rRNA gene pyrosequencing. In: FEMS Immunology and Medical Microbiology. 2011 ; Vol. 63, No. 3. pp. 397-406.
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