Ability of various alkylating agents to induce adaptive and SOS responses: A study with lacZ fusion

Masanori Otsuka, usaku Nakabeppu, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We used alkA′-lacZ′ and umuC′-lacZ′ fused genes and determined the ability of various alkylating agents to induce adaptive and SOS responses. The degree of induction of expression of these genes was quantitatively measured by a simple colorimetric assay of ß-galactosidase activity. SN1 type methylating agents, such as N-methyl-N′-nitro-N-nitrosoguanidine and B-methyl-N-nitrosourea, were more effective inducers for the alkA than for the umuC system, while SN1 type ethylating agents, such as N-ethyl-N′-nitro-N-nitrosoguanidine and N-ethyl-N-nitrosourea, were more potent inducers for the umuC than for the alkA system. Similar but less striking effects on the two systems were obtained with SN2 type alkylating agents.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalMutation Research DNA Repair Reports
Volume146
Issue number2
DOIs
Publication statusPublished - Sep 1985

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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