Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair

Keigo Yoshizaki, Lizhi Hu, Thai Nguyen, Kiyoshi Sakai, Bing He, Chak Fong, Yoshihiko Yamada, Daniel D. Bikle, Yuko Oda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cell fates are determined by specific transcriptional programs. Here we provide evidence that the transcriptional coactivator, Mediator 1 (Med1), is essential for the cell fate determination of ectodermal epithelia. Conditional deletion of Med1 in vivo converted dental epithelia into epidermal epithelia, causing defects in enamel organ development while promoting hair formation in the incisors. We identified multiple processes by which hairs are generated in Med1 deficient incisors: 1) dental epithelial stem cells lacking Med 1 fail to commit to the dental lineage, 2) Sox2-expressing stem cells extend into the differentiation zone and remain multi-potent due to reduced Notch1 signaling, and 3) epidermal fate is induced by calcium as demonstrated in dental epithelial cell cultures. These results demonstrate that Med1 is a master regulator in adult stem cells to govern epithelial cell fate.

Original languageEnglish
Article numbere99991
JournalPloS one
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 20 2014
Externally publishedYes

Fingerprint

Ablation
Stem cells
hairs
Tooth
teeth
epithelium
Epithelium
Switches
epithelial cells
Epithelial Cells
Incisor
stem cells
Stem Cells
Enamels
Enamel Organ
cells
Cell culture
Adult Stem Cells
enamel
Calcium

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair. / Yoshizaki, Keigo; Hu, Lizhi; Nguyen, Thai; Sakai, Kiyoshi; He, Bing; Fong, Chak; Yamada, Yoshihiko; Bikle, Daniel D.; Oda, Yuko.

In: PloS one, Vol. 9, No. 6, e99991, 20.06.2014.

Research output: Contribution to journalArticle

Yoshizaki, K, Hu, L, Nguyen, T, Sakai, K, He, B, Fong, C, Yamada, Y, Bikle, DD & Oda, Y 2014, 'Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair', PloS one, vol. 9, no. 6, e99991. https://doi.org/10.1371/journal.pone.0099991
Yoshizaki, Keigo ; Hu, Lizhi ; Nguyen, Thai ; Sakai, Kiyoshi ; He, Bing ; Fong, Chak ; Yamada, Yoshihiko ; Bikle, Daniel D. ; Oda, Yuko. / Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair. In: PloS one. 2014 ; Vol. 9, No. 6.
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