Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS

Kenichi Hirano, Tomohiro Ohgomori, Kazuyoshi Kobayashi, Fumiaki Tanaka, Tomohiro Matsumoto, Takamitsu Natori, Yukihiro Matsuyama, Kenji Uchimura, Kazuma Sakamoto, Hideyuki Takeuchi, Akihiro Hirakawa, Akio Suzumura, Gen Sobue, Naoki Ishiguro, Shiro Imagama, Kenji Kadomatsu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Biopolymers consist of three major classes, i.e., polynucleotides (DNA, RNA), polypeptides (proteins) and polysaccharides (sugar chains). It is widely accepted that polynucleotides and polypeptides play fundamental roles in the pathogenesis of neurodegenerative diseases. But, sugar chains have been poorly studied in this process, and their biological/clinical significance remains largely unexplored. Amyotrophic lateral sclerosis (ALS) is a motoneuron-degenerative disease, the pathogenesis of which requires both cell autonomous and non-cell autonomous processes. Here, we investigated the role of keratan sulfate (KS), a sulfated long sugar chain of proteoglycan, in ALS pathogenesis. We employed ALS model SOD1 G93A mice and GlcNAc6ST-1 -/- mice, which are KS-deficient in the central nervous system. Unexpectedly, SOD1 G93A GlcNAc6ST-1 -/- mice exhibited a significantly shorter lifespan than SOD1 G93A mice and an accelerated appearance of clinical symptoms (body weight loss and decreased rotarod performance). KS expression was induced exclusively in a subpopulation of microglia in SOD1 G93A mice, and became detectable around motoneurons in the ventral horn during the early disease phase before body weight loss. During this phase, the expression of M2 microglia markers was transiently enhanced in SOD1 G93A mice, while this enhancement was attenuated in SOD1 G93A GlcNAc6ST-1 -/- mice. Consistent with this, M2 microglia were markedly less during the early disease phase in SOD1 G93A GlcNAc6ST-1 -/- mice. Moreover, KS expression in microglia was also detected in some human ALS cases. This study suggests that KS plays an indispensable, suppressive role in the early phase pathogenesis of ALS and may represent a new target for therapeutic intervention.

Original languageEnglish
Article numbere66969
JournalPloS one
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 25 2013
Externally publishedYes

Fingerprint

Keratan Sulfate
Amyotrophic Lateral Sclerosis
Ablation
sulfates
pathogenesis
Sugars
mice
neuroglia
Microglia
Polynucleotides
polynucleotides
Neurodegenerative diseases
Motor Neurons
motor neurons
sugars
Peptides
Biopolymers
Neurology
Weight Loss
Proteoglycans

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hirano, K., Ohgomori, T., Kobayashi, K., Tanaka, F., Matsumoto, T., Natori, T., ... Kadomatsu, K. (2013). Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS. PloS one, 8(6), [e66969]. https://doi.org/10.1371/journal.pone.0066969

Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS. / Hirano, Kenichi; Ohgomori, Tomohiro; Kobayashi, Kazuyoshi; Tanaka, Fumiaki; Matsumoto, Tomohiro; Natori, Takamitsu; Matsuyama, Yukihiro; Uchimura, Kenji; Sakamoto, Kazuma; Takeuchi, Hideyuki; Hirakawa, Akihiro; Suzumura, Akio; Sobue, Gen; Ishiguro, Naoki; Imagama, Shiro; Kadomatsu, Kenji.

In: PloS one, Vol. 8, No. 6, e66969, 25.06.2013.

Research output: Contribution to journalArticle

Hirano, K, Ohgomori, T, Kobayashi, K, Tanaka, F, Matsumoto, T, Natori, T, Matsuyama, Y, Uchimura, K, Sakamoto, K, Takeuchi, H, Hirakawa, A, Suzumura, A, Sobue, G, Ishiguro, N, Imagama, S & Kadomatsu, K 2013, 'Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS', PloS one, vol. 8, no. 6, e66969. https://doi.org/10.1371/journal.pone.0066969
Hirano K, Ohgomori T, Kobayashi K, Tanaka F, Matsumoto T, Natori T et al. Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS. PloS one. 2013 Jun 25;8(6). e66969. https://doi.org/10.1371/journal.pone.0066969
Hirano, Kenichi ; Ohgomori, Tomohiro ; Kobayashi, Kazuyoshi ; Tanaka, Fumiaki ; Matsumoto, Tomohiro ; Natori, Takamitsu ; Matsuyama, Yukihiro ; Uchimura, Kenji ; Sakamoto, Kazuma ; Takeuchi, Hideyuki ; Hirakawa, Akihiro ; Suzumura, Akio ; Sobue, Gen ; Ishiguro, Naoki ; Imagama, Shiro ; Kadomatsu, Kenji. / Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS. In: PloS one. 2013 ; Vol. 8, No. 6.
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