Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration

Chihiro Ueyama, Hiroki Akashiba, Keiko Nakayama, Keiichi Nakayama, Nobuyoshi Nishiyama, Norio Matsuki

Research output: Contribution to journalArticle

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Abstract

p27 is a cyclin-dependent kinase inhibitor which arrests cell cycle at G1-S phase. Using RNA interference method, we previously showed that reduction of endogenous p27 expression induces cell death through cell cycle progression in cultured cortical neurons. In this study, we investigated responses to kainate treatment using p27 knockout mice. Injection of kainic acid induced p27 downregulation and retinoblastoma protein phosphorylation in wild-type mouse hippocampus. No change was observed in hippocampal cell viability in untreated adult p27 heterozygous and homozygous mice compared with wild type (+/+). p27 homozygous mice, however, displayed enhanced seizure and hippocampal degeneration after kainic acid treatment. This study first suggests that ablation of p27 enhance kainate-induced seizure and hippocampal cell death in vivo.

Original languageEnglish
Pages (from-to)1781-1785
Number of pages5
JournalNeuroReport
Volume18
Issue number17
DOIs
Publication statusPublished - Nov 2007

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Kainic Acid
Seizures
Cell Death
Retinoblastoma Protein
Cyclin-Dependent Kinases
G1 Phase
RNA Interference
Cell Cycle Checkpoints
S Phase
Knockout Mice
Hippocampus
Cell Survival
Cell Cycle
Down-Regulation
Phosphorylation
Neurons
Injections
Therapeutics

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Ueyama, C., Akashiba, H., Nakayama, K., Nakayama, K., Nishiyama, N., & Matsuki, N. (2007). Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration. NeuroReport, 18(17), 1781-1785. https://doi.org/10.1097/WNR.0b013e3282f16df6

Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration. / Ueyama, Chihiro; Akashiba, Hiroki; Nakayama, Keiko; Nakayama, Keiichi; Nishiyama, Nobuyoshi; Matsuki, Norio.

In: NeuroReport, Vol. 18, No. 17, 11.2007, p. 1781-1785.

Research output: Contribution to journalArticle

Ueyama, C, Akashiba, H, Nakayama, K, Nakayama, K, Nishiyama, N & Matsuki, N 2007, 'Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration', NeuroReport, vol. 18, no. 17, pp. 1781-1785. https://doi.org/10.1097/WNR.0b013e3282f16df6
Ueyama, Chihiro ; Akashiba, Hiroki ; Nakayama, Keiko ; Nakayama, Keiichi ; Nishiyama, Nobuyoshi ; Matsuki, Norio. / Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration. In: NeuroReport. 2007 ; Vol. 18, No. 17. pp. 1781-1785.
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