Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice

Tatsuo Furuyama, Kazuko Kitayama, Yuri Shimoda, Minetaro Ogawa, Kiyoaki Sone, Kiyomi Yoshida-Araki, Hiroshi Hisatsune, Shin Ichi Nishikawa, Keiko Nakayama, Keiichi Nakayama, Kyoji Ikeda, Noboru Motoyama, Nozomu Mori

Research output: Contribution to journalArticle

239 Citations (Scopus)

Abstract

Members of the Foxo family, Foxo1 (Fkhr), Foxo3 (Fkhr11), and Foxo4 (Afx), are mammalian homologs of daf-16, which influences life span and energy metabolism in Caenorhabditis elegans. Mammalian FOXO proteins also play important roles in cell cycle arrest, apoptosis, stress resistance, and energy metabolism. In this study, we generated Foxo1-deficient mice to investigate the physiological role of FOXO1. The Foxo1-deficient mice died around embryonic day 11 because of defects in the branchial arches and remarkably impaired vascular development of embryos and yolk sacs. In vitro differentiation of embryonic stem cells demonstrated that endothelial cells derived from wild-type and Foxo1-deficient embryonic stem cells were able to produce comparable numbers of colonies supported by a layer of OP9 stromal cells. Although the morphology of the endothelial cell colonies was identical in both genotypes in the absence of exogenous vascular endothelial growth factor (VEGF), Foxo1-deficient endothelial cells showed a markedly different morphological response compared with wild-type endothelial cells in the presence of exogenous VEGF. These results suggest that Foxo1 is essential to the ability of endothelial cells to respond properly to a high dose of VEGF, thereby playing a critical role in normal vascular development.

Original languageEnglish
Pages (from-to)34741-34749
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number33
DOIs
Publication statusPublished - Aug 13 2004

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Endothelial cells
Endothelial Cells
Vascular Endothelial Growth Factor A
Embryonic Stem Cells
Stem cells
Energy Metabolism
Blood Vessels
Branchial Region
Yolk Sac
Aptitude
Caenorhabditis elegans
Arches
Stromal Cells
Cell Cycle Checkpoints
Embryonic Development
Genotype
Cells
Apoptosis
Defects
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Furuyama, T., Kitayama, K., Shimoda, Y., Ogawa, M., Sone, K., Yoshida-Araki, K., ... Mori, N. (2004). Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice. Journal of Biological Chemistry, 279(33), 34741-34749. https://doi.org/10.1074/jbc.M314214200

Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice. / Furuyama, Tatsuo; Kitayama, Kazuko; Shimoda, Yuri; Ogawa, Minetaro; Sone, Kiyoaki; Yoshida-Araki, Kiyomi; Hisatsune, Hiroshi; Nishikawa, Shin Ichi; Nakayama, Keiko; Nakayama, Keiichi; Ikeda, Kyoji; Motoyama, Noboru; Mori, Nozomu.

In: Journal of Biological Chemistry, Vol. 279, No. 33, 13.08.2004, p. 34741-34749.

Research output: Contribution to journalArticle

Furuyama, T, Kitayama, K, Shimoda, Y, Ogawa, M, Sone, K, Yoshida-Araki, K, Hisatsune, H, Nishikawa, SI, Nakayama, K, Nakayama, K, Ikeda, K, Motoyama, N & Mori, N 2004, 'Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice', Journal of Biological Chemistry, vol. 279, no. 33, pp. 34741-34749. https://doi.org/10.1074/jbc.M314214200
Furuyama T, Kitayama K, Shimoda Y, Ogawa M, Sone K, Yoshida-Araki K et al. Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice. Journal of Biological Chemistry. 2004 Aug 13;279(33):34741-34749. https://doi.org/10.1074/jbc.M314214200
Furuyama, Tatsuo ; Kitayama, Kazuko ; Shimoda, Yuri ; Ogawa, Minetaro ; Sone, Kiyoaki ; Yoshida-Araki, Kiyomi ; Hisatsune, Hiroshi ; Nishikawa, Shin Ichi ; Nakayama, Keiko ; Nakayama, Keiichi ; Ikeda, Kyoji ; Motoyama, Noboru ; Mori, Nozomu. / Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 33. pp. 34741-34749.
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