Abnormal arrangement of a collagen/apatite extracellular matrix orthogonal to osteoblast alignment is constructed by a nanoscale periodic surface structure

Aira Matsugaki, Gento Aramoto, Takafumi Ninomiya, Hiroshi Sawada, Satoshi Hata, Takayoshi Nakano

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Morphological and directional alteration of cells is essential for structurally appropriate construction of tissues and organs. In particular, osteoblast alignment is crucial for the realization of anisotropic bone tissue microstructure. In this article, the orientation of a collagen/apatite extracellular matrix (ECM) was established by controlling osteoblast alignment using a surface geometry with nanometer-sized periodicity induced by laser ablation. Laser irradiation induced self-organized periodic structures (laser-induced periodic surface structures; LIPSS) with a spatial period equal to the wavelength of the incident laser on the surface of biomedical alloys of Ti-6Al-4V and Co-Cr-Mo. Osteoblast orientation was successfully induced parallel to the grating structure. Notably, both the fibrous orientation of the secreted collagen matrix and the c-axis of the produced apatite crystals were orientated orthogonal to the cell direction. To the best of our knowledge, this is the first report demonstrating that bone tissue anisotropy is controllable, including the characteristic organization of a collagen/apatite composite orthogonal to the osteoblast orientation, by controlling the cell alignment using periodic surface geometry.

Original languageEnglish
Pages (from-to)134-143
Number of pages10
JournalBiomaterials
Volume37
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Apatites
Osteoblasts
Apatite
Collagen
Surface structure
Extracellular Matrix
Lasers
Tissue
Bone
Bone and Bones
Geometry
Periodic structures
Anisotropy
Laser Therapy
Periodicity
Laser ablation
Laser beam effects
Wavelength
Crystals
Microstructure

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Abnormal arrangement of a collagen/apatite extracellular matrix orthogonal to osteoblast alignment is constructed by a nanoscale periodic surface structure. / Matsugaki, Aira; Aramoto, Gento; Ninomiya, Takafumi; Sawada, Hiroshi; Hata, Satoshi; Nakano, Takayoshi.

In: Biomaterials, Vol. 37, 01.01.2015, p. 134-143.

Research output: Contribution to journalArticle

Matsugaki, Aira ; Aramoto, Gento ; Ninomiya, Takafumi ; Sawada, Hiroshi ; Hata, Satoshi ; Nakano, Takayoshi. / Abnormal arrangement of a collagen/apatite extracellular matrix orthogonal to osteoblast alignment is constructed by a nanoscale periodic surface structure. In: Biomaterials. 2015 ; Vol. 37. pp. 134-143.
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