Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects

Seiji Takashima, Masanori Takehashi, Jiyoung Lee, Shinichiro Chuma, Masaki Okano, Kenichiro Hata, Isao Suetake, Norio Nakatsuji, Hiroyuki Miyoshi, Shoji Tajima, Yoriko Tanaka, Shinya Toyokuni, Hiroyuki Sasaki, Mito Kanatsu-Shinohara, Takashi Shinohara

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Although spermatogonial stem cells (SSCs) are committed to spermatogenesis, they may also convert to an embryonic stem cell-like pluripotent state at a low frequency. Because changes in DNA methylation patterns are associated with this conversion, we examined the effect of manipulating DNA methyltransferase (Dnmt) expression on the fate of cultured SSCs, germline stem (GS) cells. Dnmt1 knockdown induced apoptosis in GS cells, which was attenuated by the loss of Trp53. In contrast, GS cells proliferated normally in vitro after Dnmt3a/Dnmt3b ablation or during Dnmt3l overexpression. However, Dnmt3a/Dnmt3b double-mutant cells showed hypomethylation in the SineB1 repetitive sequence, and Dnmt3l-overexpressing cells showed hypermethylation in major and minor satellite sequences; neither cell type formed teratomas and completed spermatogenesis following transplantation into the seminiferous tubules. Although genetic manipulation did not increase the conversion of GS cells to a pluripotent state, these results underscore the important role of DNMTs in survival and spermatogenic differentiation in SSCs.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalBiology of Reproduction
Volume81
Issue number1
DOIs
Publication statusPublished - Jul 1 2009
Externally publishedYes

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Methyltransferases
Stem Cells
DNA
Spermatogenesis
Seminiferous Tubules
Nucleic Acid Repetitive Sequences
Teratoma
DNA Methylation
Embryonic Stem Cells
Transplantation
Apoptosis

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

Cite this

Takashima, S., Takehashi, M., Lee, J., Chuma, S., Okano, M., Hata, K., ... Shinohara, T. (2009). Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects. Biology of Reproduction, 81(1), 155-164. https://doi.org/10.1095/biolreprod.108.074708

Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects. / Takashima, Seiji; Takehashi, Masanori; Lee, Jiyoung; Chuma, Shinichiro; Okano, Masaki; Hata, Kenichiro; Suetake, Isao; Nakatsuji, Norio; Miyoshi, Hiroyuki; Tajima, Shoji; Tanaka, Yoriko; Toyokuni, Shinya; Sasaki, Hiroyuki; Kanatsu-Shinohara, Mito; Shinohara, Takashi.

In: Biology of Reproduction, Vol. 81, No. 1, 01.07.2009, p. 155-164.

Research output: Contribution to journalArticle

Takashima, S, Takehashi, M, Lee, J, Chuma, S, Okano, M, Hata, K, Suetake, I, Nakatsuji, N, Miyoshi, H, Tajima, S, Tanaka, Y, Toyokuni, S, Sasaki, H, Kanatsu-Shinohara, M & Shinohara, T 2009, 'Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects', Biology of Reproduction, vol. 81, no. 1, pp. 155-164. https://doi.org/10.1095/biolreprod.108.074708
Takashima, Seiji ; Takehashi, Masanori ; Lee, Jiyoung ; Chuma, Shinichiro ; Okano, Masaki ; Hata, Kenichiro ; Suetake, Isao ; Nakatsuji, Norio ; Miyoshi, Hiroyuki ; Tajima, Shoji ; Tanaka, Yoriko ; Toyokuni, Shinya ; Sasaki, Hiroyuki ; Kanatsu-Shinohara, Mito ; Shinohara, Takashi. / Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects. In: Biology of Reproduction. 2009 ; Vol. 81, No. 1. pp. 155-164.
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