Abnormal hematopoiesis and autoimmunity in human subjects with germline IKZF1 mutations

Akihiro Hoshino, Satoshi Okada, Kenichi Yoshida, Naonori Nishida, Yusuke Okuno, Hiroo Ueno, Motoi Yamashita, Tsubasa Okano, Miyuki Tsumura, Shiho Nishimura, Sonoko Sakata, Masao Kobayashi, Haruna Nakamura, Junji Kamizono, Kanako Mitsui-Sekinaka, Takuya Ichimura, Shoichi Ohga, Yozo Nakazawa, Masatoshi Takagi, Kohsuke Imai & 9 others Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru Miyano, Seishi Ogawa, Seiji Kojima, Shigeaki Nonoyama, Tomohiro Morio, Hirokazu Kanegane

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background Ikaros, which is encoded by IKZF1, is a transcriptional factor that play a critical role in hematopoiesis. Somatic IKZF1 alterations are known to be involved in the pathogenesis of leukemia in human subjects. Recently, immunodeficiency caused by germline IKZF1 mutation has been described. Objective We sought to describe the clinical and immunologic phenotypes of Japanese patients with heterozygous IKZF1 mutations. Methods We performed whole-exome sequencing in patients from a dysgammaglobulinemia or autoimmune disease cohort and used a candidate gene approach in 4 patients. Functional and laboratory studies, including detailed lymphopoiesis/hematopoiesis analysis in the bone marrow, were performed. Results Nine patients from 6 unrelated families were identified to have heterozygous germline mutations in IKZF1. Age of onset was 0 to 20 years (mean, 7.4 years). Eight of 9 patients presented with dysgammaglobulinemia accompanied by B-cell deficiency. Four of 9 patients had autoimmune disease, including immune thrombocytopenic purpura, IgA vasculitis, and systemic lupus erythematosus. Nonautoimmune pancytopenia was observed in 1 patient. All of the mutant Ikaros protein demonstrated impaired DNA binding to the target sequence and abnormal diffuse nuclear localization. Flow cytometric analysis of bone marrow revealed reduced levels of common lymphoid progenitors and normal development of pro-B to pre-B cells. Conclusions Germline heterozygous IKZF1 mutations cause dysgammaglobulinemia; hematologic abnormalities, including B-cell defect; and autoimmune diseases.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume140
Issue number1
DOIs
Publication statusPublished - Jul 1 2017
Externally publishedYes

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Germ-Line Mutation
Hematopoiesis
Autoimmunity
Dysgammaglobulinemia
Autoimmune Diseases
Ikaros Transcription Factor
B-Lymphocytes
Lymphoid Progenitor Cells
Bone Marrow
Lymphopoiesis
Exome
Pancytopenia
Mutation
B-Lymphoid Precursor Cells
Idiopathic Thrombocytopenic Purpura
Mutant Proteins
Vasculitis
Age of Onset
Systemic Lupus Erythematosus
Immunoglobulin A

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Hoshino, A., Okada, S., Yoshida, K., Nishida, N., Okuno, Y., Ueno, H., ... Kanegane, H. (2017). Abnormal hematopoiesis and autoimmunity in human subjects with germline IKZF1 mutations. Journal of Allergy and Clinical Immunology, 140(1), 223-231. https://doi.org/10.1016/j.jaci.2016.09.029

Abnormal hematopoiesis and autoimmunity in human subjects with germline IKZF1 mutations. / Hoshino, Akihiro; Okada, Satoshi; Yoshida, Kenichi; Nishida, Naonori; Okuno, Yusuke; Ueno, Hiroo; Yamashita, Motoi; Okano, Tsubasa; Tsumura, Miyuki; Nishimura, Shiho; Sakata, Sonoko; Kobayashi, Masao; Nakamura, Haruna; Kamizono, Junji; Mitsui-Sekinaka, Kanako; Ichimura, Takuya; Ohga, Shoichi; Nakazawa, Yozo; Takagi, Masatoshi; Imai, Kohsuke; Shiraishi, Yuichi; Chiba, Kenichi; Tanaka, Hiroko; Miyano, Satoru; Ogawa, Seishi; Kojima, Seiji; Nonoyama, Shigeaki; Morio, Tomohiro; Kanegane, Hirokazu.

In: Journal of Allergy and Clinical Immunology, Vol. 140, No. 1, 01.07.2017, p. 223-231.

Research output: Contribution to journalArticle

Hoshino, A, Okada, S, Yoshida, K, Nishida, N, Okuno, Y, Ueno, H, Yamashita, M, Okano, T, Tsumura, M, Nishimura, S, Sakata, S, Kobayashi, M, Nakamura, H, Kamizono, J, Mitsui-Sekinaka, K, Ichimura, T, Ohga, S, Nakazawa, Y, Takagi, M, Imai, K, Shiraishi, Y, Chiba, K, Tanaka, H, Miyano, S, Ogawa, S, Kojima, S, Nonoyama, S, Morio, T & Kanegane, H 2017, 'Abnormal hematopoiesis and autoimmunity in human subjects with germline IKZF1 mutations', Journal of Allergy and Clinical Immunology, vol. 140, no. 1, pp. 223-231. https://doi.org/10.1016/j.jaci.2016.09.029
Hoshino, Akihiro ; Okada, Satoshi ; Yoshida, Kenichi ; Nishida, Naonori ; Okuno, Yusuke ; Ueno, Hiroo ; Yamashita, Motoi ; Okano, Tsubasa ; Tsumura, Miyuki ; Nishimura, Shiho ; Sakata, Sonoko ; Kobayashi, Masao ; Nakamura, Haruna ; Kamizono, Junji ; Mitsui-Sekinaka, Kanako ; Ichimura, Takuya ; Ohga, Shoichi ; Nakazawa, Yozo ; Takagi, Masatoshi ; Imai, Kohsuke ; Shiraishi, Yuichi ; Chiba, Kenichi ; Tanaka, Hiroko ; Miyano, Satoru ; Ogawa, Seishi ; Kojima, Seiji ; Nonoyama, Shigeaki ; Morio, Tomohiro ; Kanegane, Hirokazu. / Abnormal hematopoiesis and autoimmunity in human subjects with germline IKZF1 mutations. In: Journal of Allergy and Clinical Immunology. 2017 ; Vol. 140, No. 1. pp. 223-231.
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abstract = "Background Ikaros, which is encoded by IKZF1, is a transcriptional factor that play a critical role in hematopoiesis. Somatic IKZF1 alterations are known to be involved in the pathogenesis of leukemia in human subjects. Recently, immunodeficiency caused by germline IKZF1 mutation has been described. Objective We sought to describe the clinical and immunologic phenotypes of Japanese patients with heterozygous IKZF1 mutations. Methods We performed whole-exome sequencing in patients from a dysgammaglobulinemia or autoimmune disease cohort and used a candidate gene approach in 4 patients. Functional and laboratory studies, including detailed lymphopoiesis/hematopoiesis analysis in the bone marrow, were performed. Results Nine patients from 6 unrelated families were identified to have heterozygous germline mutations in IKZF1. Age of onset was 0 to 20 years (mean, 7.4 years). Eight of 9 patients presented with dysgammaglobulinemia accompanied by B-cell deficiency. Four of 9 patients had autoimmune disease, including immune thrombocytopenic purpura, IgA vasculitis, and systemic lupus erythematosus. Nonautoimmune pancytopenia was observed in 1 patient. All of the mutant Ikaros protein demonstrated impaired DNA binding to the target sequence and abnormal diffuse nuclear localization. Flow cytometric analysis of bone marrow revealed reduced levels of common lymphoid progenitors and normal development of pro-B to pre-B cells. Conclusions Germline heterozygous IKZF1 mutations cause dysgammaglobulinemia; hematologic abnormalities, including B-cell defect; and autoimmune diseases.",
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T1 - Abnormal hematopoiesis and autoimmunity in human subjects with germline IKZF1 mutations

AU - Hoshino, Akihiro

AU - Okada, Satoshi

AU - Yoshida, Kenichi

AU - Nishida, Naonori

AU - Okuno, Yusuke

AU - Ueno, Hiroo

AU - Yamashita, Motoi

AU - Okano, Tsubasa

AU - Tsumura, Miyuki

AU - Nishimura, Shiho

AU - Sakata, Sonoko

AU - Kobayashi, Masao

AU - Nakamura, Haruna

AU - Kamizono, Junji

AU - Mitsui-Sekinaka, Kanako

AU - Ichimura, Takuya

AU - Ohga, Shoichi

AU - Nakazawa, Yozo

AU - Takagi, Masatoshi

AU - Imai, Kohsuke

AU - Shiraishi, Yuichi

AU - Chiba, Kenichi

AU - Tanaka, Hiroko

AU - Miyano, Satoru

AU - Ogawa, Seishi

AU - Kojima, Seiji

AU - Nonoyama, Shigeaki

AU - Morio, Tomohiro

AU - Kanegane, Hirokazu

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Background Ikaros, which is encoded by IKZF1, is a transcriptional factor that play a critical role in hematopoiesis. Somatic IKZF1 alterations are known to be involved in the pathogenesis of leukemia in human subjects. Recently, immunodeficiency caused by germline IKZF1 mutation has been described. Objective We sought to describe the clinical and immunologic phenotypes of Japanese patients with heterozygous IKZF1 mutations. Methods We performed whole-exome sequencing in patients from a dysgammaglobulinemia or autoimmune disease cohort and used a candidate gene approach in 4 patients. Functional and laboratory studies, including detailed lymphopoiesis/hematopoiesis analysis in the bone marrow, were performed. Results Nine patients from 6 unrelated families were identified to have heterozygous germline mutations in IKZF1. Age of onset was 0 to 20 years (mean, 7.4 years). Eight of 9 patients presented with dysgammaglobulinemia accompanied by B-cell deficiency. Four of 9 patients had autoimmune disease, including immune thrombocytopenic purpura, IgA vasculitis, and systemic lupus erythematosus. Nonautoimmune pancytopenia was observed in 1 patient. All of the mutant Ikaros protein demonstrated impaired DNA binding to the target sequence and abnormal diffuse nuclear localization. Flow cytometric analysis of bone marrow revealed reduced levels of common lymphoid progenitors and normal development of pro-B to pre-B cells. Conclusions Germline heterozygous IKZF1 mutations cause dysgammaglobulinemia; hematologic abnormalities, including B-cell defect; and autoimmune diseases.

AB - Background Ikaros, which is encoded by IKZF1, is a transcriptional factor that play a critical role in hematopoiesis. Somatic IKZF1 alterations are known to be involved in the pathogenesis of leukemia in human subjects. Recently, immunodeficiency caused by germline IKZF1 mutation has been described. Objective We sought to describe the clinical and immunologic phenotypes of Japanese patients with heterozygous IKZF1 mutations. Methods We performed whole-exome sequencing in patients from a dysgammaglobulinemia or autoimmune disease cohort and used a candidate gene approach in 4 patients. Functional and laboratory studies, including detailed lymphopoiesis/hematopoiesis analysis in the bone marrow, were performed. Results Nine patients from 6 unrelated families were identified to have heterozygous germline mutations in IKZF1. Age of onset was 0 to 20 years (mean, 7.4 years). Eight of 9 patients presented with dysgammaglobulinemia accompanied by B-cell deficiency. Four of 9 patients had autoimmune disease, including immune thrombocytopenic purpura, IgA vasculitis, and systemic lupus erythematosus. Nonautoimmune pancytopenia was observed in 1 patient. All of the mutant Ikaros protein demonstrated impaired DNA binding to the target sequence and abnormal diffuse nuclear localization. Flow cytometric analysis of bone marrow revealed reduced levels of common lymphoid progenitors and normal development of pro-B to pre-B cells. Conclusions Germline heterozygous IKZF1 mutations cause dysgammaglobulinemia; hematologic abnormalities, including B-cell defect; and autoimmune diseases.

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