Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice

Shoichi Ohga, Y. Yoshikai, K. Matsumoto, K. Kishihara, G. Matsuzaki, K. Nomoto

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Abstract

To search the abnormality in prethymic T-cell precursors in lpr/lpr(lpr) mice, rearrangement and expression of T-cell receptor (TcR) genes were investigated in long-term cultured bone marrow (LTBM) cells of lpr mice, in which the developmental steps of T-cell precursors may be better synchronized than those in bone marrow (BM) cells. Neither rearrangement nor expression of TCR γ and δ genes were detected in the LTBM cells from +/+ control mice, whereas some γ gene rearrangements were detected in those derived from lpr mice, irrespective of the genetic background. When BM cells or LTBM cells from lpr mice were transplanted into supralethally irradiated +/+ mice, the lpr-derived BM cells appeared earlier in the thymus of the recipient mice than +/+-derived BM cells and the recipients suffered from lethal wasting syndrome. In addition, the lpr-derived BM cells showed higher activity in colony-forming unit spleen (CFUs) than the +/+-derived BM cells. These results suggest that the T-cell progenitors in the BM of lpr mice may be different not only in quantity but also in quality from those of +/+ mice.

Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalImmunology
Volume67
Issue number4
Publication statusPublished - 1989

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Ohga, S., Yoshikai, Y., Matsumoto, K., Kishihara, K., Matsuzaki, G., & Nomoto, K. (1989). Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice. Immunology, 67(4), 543-546.