Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice

Shoichi Ohga, Y. Yoshikai, K. Matsumoto, K. Kishihara, G. Matsuzaki, K. Nomoto

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Abstract

To search the abnormality in prethymic T-cell precursors in lpr/lpr(lpr) mice, rearrangement and expression of T-cell receptor (TcR) genes were investigated in long-term cultured bone marrow (LTBM) cells of lpr mice, in which the developmental steps of T-cell precursors may be better synchronized than those in bone marrow (BM) cells. Neither rearrangement nor expression of TCR γ and δ genes were detected in the LTBM cells from +/+ control mice, whereas some γ gene rearrangements were detected in those derived from lpr mice, irrespective of the genetic background. When BM cells or LTBM cells from lpr mice were transplanted into supralethally irradiated +/+ mice, the lpr-derived BM cells appeared earlier in the thymus of the recipient mice than +/+-derived BM cells and the recipients suffered from lethal wasting syndrome. In addition, the lpr-derived BM cells showed higher activity in colony-forming unit spleen (CFUs) than the +/+-derived BM cells. These results suggest that the T-cell progenitors in the BM of lpr mice may be different not only in quantity but also in quality from those of +/+ mice.

Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalImmunology
Volume67
Issue number4
Publication statusPublished - 1989

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T-Cell Receptor Genes
Bone Marrow Cells
Cultured Cells
T-Lymphoid Precursor Cells
Wasting Syndrome
Gene Rearrangement
Thymus Gland
Stem Cells
Spleen
Bone Marrow
T-Lymphocytes
Gene Expression

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Ohga, S., Yoshikai, Y., Matsumoto, K., Kishihara, K., Matsuzaki, G., & Nomoto, K. (1989). Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice. Immunology, 67(4), 543-546.

Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice. / Ohga, Shoichi; Yoshikai, Y.; Matsumoto, K.; Kishihara, K.; Matsuzaki, G.; Nomoto, K.

In: Immunology, Vol. 67, No. 4, 1989, p. 543-546.

Research output: Contribution to journalArticle

Ohga, S, Yoshikai, Y, Matsumoto, K, Kishihara, K, Matsuzaki, G & Nomoto, K 1989, 'Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice', Immunology, vol. 67, no. 4, pp. 543-546.
Ohga S, Yoshikai Y, Matsumoto K, Kishihara K, Matsuzaki G, Nomoto K. Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice. Immunology. 1989;67(4):543-546.
Ohga, Shoichi ; Yoshikai, Y. ; Matsumoto, K. ; Kishihara, K. ; Matsuzaki, G. ; Nomoto, K. / Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice. In: Immunology. 1989 ; Vol. 67, No. 4. pp. 543-546.
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