Abnormalities of stromal structure in the bullous keratopathy cornea identified by second harmonic eneration imaging microscopy

Naoyuki Morishige, Norihiro Yamada, Xu Zhang, Yukiko Morita, Naoyuki Yamada, Kazuhiro Kimura, Atsushi Takahara, Koh Hei Sonoda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

PURPOSE. To identify structural alterations in collagen lamellae and the transdifferentiation of keratocytes into myofibroblasts in the corneal stroma of bullous keratopathy (BK) patients and to examine the relation of such changes to the duration of stromal edema or the underlying cause of BK. METHODS. Six normal human corneas and 16 BK corneas were subjected to second harmonic generation (SHG) imaging microscopy to allow three-dimensional (3-D) reconstruction of collagen lamellae. Expression of α-smooth muscle actin (αSMA) was examined by immunofluorescence analysis and conventional laser confocal microscopy. RESULTS. Collagen lamellae were interwoven at the anterior stroma and uniformly aligned at the posterior stroma, whereas aSMA was not detected throughout the entire stroma of the normal cornea. Nine (56%) and 7 (44%) of the 16 BK corneas showed abnormal collagen structure at the anterior and posterior stroma, respectively. Expression of αSMA was detected in the anterior or posterior stroma of 7 (44%) and 6 (38%) of the 16 BK corneas, respectively. Disorganization of collagen lamellae and myofibroblastic transdifferentiation were detected only in corneas with a duration of stromal edema of at least 12 months. Corneas with BK as a result of birth injury showed abnormal collagen structure at the posterior stroma, whereas those with BK resulting from laser iridotomy did not. CONCLUSIONS. Changes in the structure of the entire stroma were detected in BK corneas with a duration of stromal edema of at least 12 months, suggesting that such changes may be progressive. In addition, the underlying cause of BK may influence structural changes at the posterior stroma.

Original languageEnglish
Pages (from-to)4998-5003
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number8
DOIs
Publication statusPublished - Jul 1 2012

Fingerprint

Cornea
Microscopy
Collagen
Edema
Confocal Microscopy
Smooth Muscle
Actins
Birth Injuries
Corneal Stroma
Myofibroblasts
Fluorescent Antibody Technique
Lasers

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Abnormalities of stromal structure in the bullous keratopathy cornea identified by second harmonic eneration imaging microscopy. / Morishige, Naoyuki; Yamada, Norihiro; Zhang, Xu; Morita, Yukiko; Yamada, Naoyuki; Kimura, Kazuhiro; Takahara, Atsushi; Sonoda, Koh Hei.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 8, 01.07.2012, p. 4998-5003.

Research output: Contribution to journalArticle

Morishige, Naoyuki ; Yamada, Norihiro ; Zhang, Xu ; Morita, Yukiko ; Yamada, Naoyuki ; Kimura, Kazuhiro ; Takahara, Atsushi ; Sonoda, Koh Hei. / Abnormalities of stromal structure in the bullous keratopathy cornea identified by second harmonic eneration imaging microscopy. In: Investigative Ophthalmology and Visual Science. 2012 ; Vol. 53, No. 8. pp. 4998-5003.
@article{094b774d9bd349c49a01821260a1632b,
title = "Abnormalities of stromal structure in the bullous keratopathy cornea identified by second harmonic eneration imaging microscopy",
abstract = "PURPOSE. To identify structural alterations in collagen lamellae and the transdifferentiation of keratocytes into myofibroblasts in the corneal stroma of bullous keratopathy (BK) patients and to examine the relation of such changes to the duration of stromal edema or the underlying cause of BK. METHODS. Six normal human corneas and 16 BK corneas were subjected to second harmonic generation (SHG) imaging microscopy to allow three-dimensional (3-D) reconstruction of collagen lamellae. Expression of α-smooth muscle actin (αSMA) was examined by immunofluorescence analysis and conventional laser confocal microscopy. RESULTS. Collagen lamellae were interwoven at the anterior stroma and uniformly aligned at the posterior stroma, whereas aSMA was not detected throughout the entire stroma of the normal cornea. Nine (56{\%}) and 7 (44{\%}) of the 16 BK corneas showed abnormal collagen structure at the anterior and posterior stroma, respectively. Expression of αSMA was detected in the anterior or posterior stroma of 7 (44{\%}) and 6 (38{\%}) of the 16 BK corneas, respectively. Disorganization of collagen lamellae and myofibroblastic transdifferentiation were detected only in corneas with a duration of stromal edema of at least 12 months. Corneas with BK as a result of birth injury showed abnormal collagen structure at the posterior stroma, whereas those with BK resulting from laser iridotomy did not. CONCLUSIONS. Changes in the structure of the entire stroma were detected in BK corneas with a duration of stromal edema of at least 12 months, suggesting that such changes may be progressive. In addition, the underlying cause of BK may influence structural changes at the posterior stroma.",
author = "Naoyuki Morishige and Norihiro Yamada and Xu Zhang and Yukiko Morita and Naoyuki Yamada and Kazuhiro Kimura and Atsushi Takahara and Sonoda, {Koh Hei}",
year = "2012",
month = "7",
day = "1",
doi = "10.1167/iovs.12-10214",
language = "English",
volume = "53",
pages = "4998--5003",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "8",

}

TY - JOUR

T1 - Abnormalities of stromal structure in the bullous keratopathy cornea identified by second harmonic eneration imaging microscopy

AU - Morishige, Naoyuki

AU - Yamada, Norihiro

AU - Zhang, Xu

AU - Morita, Yukiko

AU - Yamada, Naoyuki

AU - Kimura, Kazuhiro

AU - Takahara, Atsushi

AU - Sonoda, Koh Hei

PY - 2012/7/1

Y1 - 2012/7/1

N2 - PURPOSE. To identify structural alterations in collagen lamellae and the transdifferentiation of keratocytes into myofibroblasts in the corneal stroma of bullous keratopathy (BK) patients and to examine the relation of such changes to the duration of stromal edema or the underlying cause of BK. METHODS. Six normal human corneas and 16 BK corneas were subjected to second harmonic generation (SHG) imaging microscopy to allow three-dimensional (3-D) reconstruction of collagen lamellae. Expression of α-smooth muscle actin (αSMA) was examined by immunofluorescence analysis and conventional laser confocal microscopy. RESULTS. Collagen lamellae were interwoven at the anterior stroma and uniformly aligned at the posterior stroma, whereas aSMA was not detected throughout the entire stroma of the normal cornea. Nine (56%) and 7 (44%) of the 16 BK corneas showed abnormal collagen structure at the anterior and posterior stroma, respectively. Expression of αSMA was detected in the anterior or posterior stroma of 7 (44%) and 6 (38%) of the 16 BK corneas, respectively. Disorganization of collagen lamellae and myofibroblastic transdifferentiation were detected only in corneas with a duration of stromal edema of at least 12 months. Corneas with BK as a result of birth injury showed abnormal collagen structure at the posterior stroma, whereas those with BK resulting from laser iridotomy did not. CONCLUSIONS. Changes in the structure of the entire stroma were detected in BK corneas with a duration of stromal edema of at least 12 months, suggesting that such changes may be progressive. In addition, the underlying cause of BK may influence structural changes at the posterior stroma.

AB - PURPOSE. To identify structural alterations in collagen lamellae and the transdifferentiation of keratocytes into myofibroblasts in the corneal stroma of bullous keratopathy (BK) patients and to examine the relation of such changes to the duration of stromal edema or the underlying cause of BK. METHODS. Six normal human corneas and 16 BK corneas were subjected to second harmonic generation (SHG) imaging microscopy to allow three-dimensional (3-D) reconstruction of collagen lamellae. Expression of α-smooth muscle actin (αSMA) was examined by immunofluorescence analysis and conventional laser confocal microscopy. RESULTS. Collagen lamellae were interwoven at the anterior stroma and uniformly aligned at the posterior stroma, whereas aSMA was not detected throughout the entire stroma of the normal cornea. Nine (56%) and 7 (44%) of the 16 BK corneas showed abnormal collagen structure at the anterior and posterior stroma, respectively. Expression of αSMA was detected in the anterior or posterior stroma of 7 (44%) and 6 (38%) of the 16 BK corneas, respectively. Disorganization of collagen lamellae and myofibroblastic transdifferentiation were detected only in corneas with a duration of stromal edema of at least 12 months. Corneas with BK as a result of birth injury showed abnormal collagen structure at the posterior stroma, whereas those with BK resulting from laser iridotomy did not. CONCLUSIONS. Changes in the structure of the entire stroma were detected in BK corneas with a duration of stromal edema of at least 12 months, suggesting that such changes may be progressive. In addition, the underlying cause of BK may influence structural changes at the posterior stroma.

UR - http://www.scopus.com/inward/record.url?scp=84867366813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867366813&partnerID=8YFLogxK

U2 - 10.1167/iovs.12-10214

DO - 10.1167/iovs.12-10214

M3 - Article

C2 - 22736613

AN - SCOPUS:84867366813

VL - 53

SP - 4998

EP - 5003

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 8

ER -