Absorption cytometric dna analysis of sections of gastric carcinomas and a comparison with cytofluorometry of single‐cell suspensions and flow cytometry

Masaru Haraguchi, Yoshihiro Kakeji, Hiroyuki Orita, Daisuke Korenaga, Yoshihiko Maehara, Keizo Sugimachi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Measurements were made of cellular deoxyribonucleic acid (DNA) content in paraffin‐embedded materials from 44 gastric carcinomas, using three different methods; static cytometric light absorbance technique using tissue sections (SCM‐abs), static cytometric fluorescence technique using single‐cell suspensions (SCM‐flu), and flow cytometry (FCM). The DNA ploidy determined by SCM‐abs was grouped into low or high ploidy according to dispersion on the DNA histogram, and the DNA ploidy determined by SCM‐flu and FCM was grouped into diploidy or aneu‐ploidy, according to the peak DNA value. Accord between SCM‐abs and SCM‐flu or between SCM‐abs and FCM was confirmed when low ploidy by SCM‐abs showed diploidy by SCM‐flu or FCM and high ploidy by SCM‐abs showed aneuploidy by SCM‐flu or FCM. An incomplete but good accord of DNA ploidy was obtained with these methods. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalJournal of Surgical Oncology
Volume60
Issue number3
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Ploidies
Stomach
Suspensions
Flow Cytometry
Carcinoma
DNA
Aneuploidy
Diploidy
Fluorescence
Light

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

Absorption cytometric dna analysis of sections of gastric carcinomas and a comparison with cytofluorometry of single‐cell suspensions and flow cytometry. / Haraguchi, Masaru; Kakeji, Yoshihiro; Orita, Hiroyuki; Korenaga, Daisuke; Maehara, Yoshihiko; Sugimachi, Keizo.

In: Journal of Surgical Oncology, Vol. 60, No. 3, 01.01.1995, p. 201-206.

Research output: Contribution to journalArticle

Haraguchi, Masaru ; Kakeji, Yoshihiro ; Orita, Hiroyuki ; Korenaga, Daisuke ; Maehara, Yoshihiko ; Sugimachi, Keizo. / Absorption cytometric dna analysis of sections of gastric carcinomas and a comparison with cytofluorometry of single‐cell suspensions and flow cytometry. In: Journal of Surgical Oncology. 1995 ; Vol. 60, No. 3. pp. 201-206.
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