Absorption of 6- O -caffeoylsophorose and its metabolites in sprague-dawley rats detected by electrochemical detector-high-performance liquid chromatography and electrospray ionization-time-of-flight-mass spectrometry methods

Ju Qiu, Nozomi Saito, Mai Noguchi, Keiichi Fukui, Kayo Yoshiyama, Kazusato Matsugano, Norihiko Terehara, Toshiro Matsui

Research output: Contribution to journalArticle

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Abstract

Absorption and metabolism of a natural compound, 6-O-caffeoylsophorose (CS) from acylated anthocyanins in a red vinegar fermented with purple sweetpotato, were clarified. The absorption of CS and conjugated CS in blood from orally administrated Sprague-Dawley rats at a dose of 400 mg/kg was investigated by electrochemical detection-high performance liquid chromatography. As a result, CS was successfully detected in rat plasma (AUC0-6h, 108.6 ± 8.1 nmol h/mL) and was found to be an intact absorbable polyphenol. In addition, half of the absorbed CS was detected as its conjugates (AUC0-6h, 50.7 ± 5.7 nmol h/mL) as well as caffeic and ferulic acids from CS. By a time-of-flight-mass spectrometric analysis of CS-administered plasma sample, glucuronide and methylated conjugates of CS were identified, in addition to glucuronide, methylated, or sulfate conjugates of caffeic and ferulic acids. Consequently, CS was absorbed in intact form into rat blood and partly degraded to caffeic and ferulic acids or metabolized by glucuronidation, methylation, or sulfatation.

Original languageEnglish
Pages (from-to)6299-6304
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number11
DOIs
Publication statusPublished - Jun 8 2011

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Electrospray ionization
High performance liquid chromatography
caffeic acid
ferulic acid
Metabolites
ionization
detectors
Mass spectrometry
Sprague Dawley Rats
Rats
Mass Spectrometry
high performance liquid chromatography
High Pressure Liquid Chromatography
mass spectrometry
metabolites
Detectors
rats
Caffeic Acids
vinegars
blood

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Absorption of 6- O -caffeoylsophorose and its metabolites in sprague-dawley rats detected by electrochemical detector-high-performance liquid chromatography and electrospray ionization-time-of-flight-mass spectrometry methods. / Qiu, Ju; Saito, Nozomi; Noguchi, Mai; Fukui, Keiichi; Yoshiyama, Kayo; Matsugano, Kazusato; Terehara, Norihiko; Matsui, Toshiro.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 11, 08.06.2011, p. 6299-6304.

Research output: Contribution to journalArticle

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abstract = "Absorption and metabolism of a natural compound, 6-O-caffeoylsophorose (CS) from acylated anthocyanins in a red vinegar fermented with purple sweetpotato, were clarified. The absorption of CS and conjugated CS in blood from orally administrated Sprague-Dawley rats at a dose of 400 mg/kg was investigated by electrochemical detection-high performance liquid chromatography. As a result, CS was successfully detected in rat plasma (AUC0-6h, 108.6 ± 8.1 nmol h/mL) and was found to be an intact absorbable polyphenol. In addition, half of the absorbed CS was detected as its conjugates (AUC0-6h, 50.7 ± 5.7 nmol h/mL) as well as caffeic and ferulic acids from CS. By a time-of-flight-mass spectrometric analysis of CS-administered plasma sample, glucuronide and methylated conjugates of CS were identified, in addition to glucuronide, methylated, or sulfate conjugates of caffeic and ferulic acids. Consequently, CS was absorbed in intact form into rat blood and partly degraded to caffeic and ferulic acids or metabolized by glucuronidation, methylation, or sulfatation.",
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AU - Terehara, Norihiko

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