TY - JOUR
T1 - Abundance of Zetaproteobacteria within crustal fluids in back-arc hydrothermal fields of the Southern Mariana Trough
AU - Kato, Shingo
AU - Yanagawa, Katsunori
AU - Sunamura, Michinari
AU - Takano, Yoshinori
AU - Ishibashi, Jun Ichiro
AU - Kakegawa, Takeshi
AU - Utsumi, Motoo
AU - Yamanaka, Toshiro
AU - Toki, Tomohiro
AU - Noguchi, Takuroh
AU - Kobayashi, Kensei
AU - Moroi, Arimichi
AU - Kimura, Hiroyuki
AU - Kawarabayasi, Yutaka
AU - Marumo, Katsumi
AU - Urabe, Tetsuro
AU - Yamagishi, Akihiko
PY - 2009/12
Y1 - 2009/12
N2 - Summary To extend knowledge of subseafloor microbial communities within the oceanic crust, the abundance, diversity and composition of microbial communities in crustal fluids at back-arc hydrothermal fields of the Southern Mariana Trough (SMT) were investigated using culture-independent molecular techniques based on 16S rRNA gene sequences. Seafloor drilling was carried out at two hydrothermal fields, on- and off-ridge of the back-arc spreading centre of the SMT. 16S rRNA gene clone libraries for bacterial and archaeal communities were constructed from the fluid samples collected from the boreholes. Phylotypes related to Thiomicrospira in the Gammaproteobacteria (putative sulfide-oxidizers) and Mariprofundus in the Zetaproteobacteria (putative iron-oxidizers) were recovered from the fluid samples. A number of unique archaeal phylotypes were also recovered. Fluorescence in situ hybridization (FISH) analysis indicated the presence of active bacterial and archaeal populations in the fluids. The Zetaproteobacteria accounted for up to 32% of the total prokaryotic cell number as shown by FISH analysis using a specific probe designed in this study. Our results lead to the hypothesis that the Zetaproteobacteria play a role in iron oxidation within the oceanic crust.
AB - Summary To extend knowledge of subseafloor microbial communities within the oceanic crust, the abundance, diversity and composition of microbial communities in crustal fluids at back-arc hydrothermal fields of the Southern Mariana Trough (SMT) were investigated using culture-independent molecular techniques based on 16S rRNA gene sequences. Seafloor drilling was carried out at two hydrothermal fields, on- and off-ridge of the back-arc spreading centre of the SMT. 16S rRNA gene clone libraries for bacterial and archaeal communities were constructed from the fluid samples collected from the boreholes. Phylotypes related to Thiomicrospira in the Gammaproteobacteria (putative sulfide-oxidizers) and Mariprofundus in the Zetaproteobacteria (putative iron-oxidizers) were recovered from the fluid samples. A number of unique archaeal phylotypes were also recovered. Fluorescence in situ hybridization (FISH) analysis indicated the presence of active bacterial and archaeal populations in the fluids. The Zetaproteobacteria accounted for up to 32% of the total prokaryotic cell number as shown by FISH analysis using a specific probe designed in this study. Our results lead to the hypothesis that the Zetaproteobacteria play a role in iron oxidation within the oceanic crust.
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U2 - 10.1111/j.1462-2920.2009.02031.x
DO - 10.1111/j.1462-2920.2009.02031.x
M3 - Article
C2 - 19691504
AN - SCOPUS:71249113173
SN - 1462-2912
VL - 11
SP - 3210
EP - 3222
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 12
ER -