Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1

Research output: Contribution to journalArticle

Abstract

Undifferentiated odontogenic epithelium and dental papilla cells differentiate into ameloblasts and odontoblasts, respectively, both of which are essential for tooth development. These differentiation processes involve dramatic functional and morphological changes of the cells. For these changes to occur, activation of mitochondrial functions, including ATP production, is extremely important. In addition, these changes are closely related to mitochondrial fission and fusion, known as mitochondrial dynamics. However, few studies have focused on the role of mitochondrial dynamics in tooth development. The purpose of this study was to clarify this role. We used mouse tooth germ organ cultures and a mouse dental papilla cell line with the ability to differentiate into odontoblasts, in combination with knockdown of the mitochondrial fission factor, dynamin related protein (DRP)1. In organ cultures of the mouse first molar, tooth germ developed to the early bell stage. The amount of dentin formed under DRP1 inhibition was significantly larger than that of the control. In experiments using a mouse dental papilla cell line, differentiation into odontoblasts was enhanced by inhibiting DRP1. This was associated with increased mitochondrial elongation and ATP production compared to the control. These results suggest that DRP1 inhibition accelerates dentin formation through mitochondrial elongation and activation. This raises the possibility that DRP1 might be a therapeutic target for developmental disorders of teeth.

Original languageEnglish
Pages (from-to)1655-1660
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume495
Issue number2
DOIs
Publication statusPublished - Jan 8 2018

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Dentinogenesis
Mitochondrial Dynamics
Dynamins
Dental Papilla
Odontoblasts
Elongation
Adenosine Triphosphate
Chemical activation
Cells
Tooth Germ
Tooth
Organ Culture Techniques
Dentin
Cell culture
Proteins
Fusion reactions
Ameloblasts
Cell Line
Cell Differentiation
Epithelium

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1",
abstract = "Undifferentiated odontogenic epithelium and dental papilla cells differentiate into ameloblasts and odontoblasts, respectively, both of which are essential for tooth development. These differentiation processes involve dramatic functional and morphological changes of the cells. For these changes to occur, activation of mitochondrial functions, including ATP production, is extremely important. In addition, these changes are closely related to mitochondrial fission and fusion, known as mitochondrial dynamics. However, few studies have focused on the role of mitochondrial dynamics in tooth development. The purpose of this study was to clarify this role. We used mouse tooth germ organ cultures and a mouse dental papilla cell line with the ability to differentiate into odontoblasts, in combination with knockdown of the mitochondrial fission factor, dynamin related protein (DRP)1. In organ cultures of the mouse first molar, tooth germ developed to the early bell stage. The amount of dentin formed under DRP1 inhibition was significantly larger than that of the control. In experiments using a mouse dental papilla cell line, differentiation into odontoblasts was enhanced by inhibiting DRP1. This was associated with increased mitochondrial elongation and ATP production compared to the control. These results suggest that DRP1 inhibition accelerates dentin formation through mitochondrial elongation and activation. This raises the possibility that DRP1 might be a therapeutic target for developmental disorders of teeth.",
author = "Matsuishi, {Yumiko I.} and Hiroki Kato and Keiji Masuda and Haruyoshi Yamaza and Yuta Hirofuji and Hiroshi Sato and Hiroko Wada and Tamotsu Kiyoshima and Kazuaki Nonaka",
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AU - Matsuishi, Yumiko I.

AU - Kato, Hiroki

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AU - Yamaza, Haruyoshi

AU - Hirofuji, Yuta

AU - Sato, Hiroshi

AU - Wada, Hiroko

AU - Kiyoshima, Tamotsu

AU - Nonaka, Kazuaki

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