Accelerated evolution of crotalinae snake venom gland serine proteases

Masanobu Deshimaru, Tomohisa Ogawa, Kinichi Nakashima, Ikuo Nobuhisa, Takahito Chijiwa, Yasuyuki Shimohigashi, Yasuyuki Fukumaki, Mineo Niwa, Ikuo Yamashina, Shosaku Hattori, Motonori Ohno

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A2 (PLA2) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalFEBS Letters
Volume397
Issue number1
DOIs
Publication statusPublished - Nov 11 1996

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Trimeresurus
Snake Venoms
Serine Proteases
Genes
Venoms
Isoenzymes
Amino Acids
Snakes
Phospholipases A2
Multigene Family
Southern Blotting
Gene Library
Open Reading Frames
Amino Acid Sequence
Substitution reactions
Nucleotides
Complementary DNA
Genome
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Deshimaru, M., Ogawa, T., Nakashima, K., Nobuhisa, I., Chijiwa, T., Shimohigashi, Y., ... Ohno, M. (1996). Accelerated evolution of crotalinae snake venom gland serine proteases. FEBS Letters, 397(1), 83-88. https://doi.org/10.1016/S0014-5793(96)01144-1

Accelerated evolution of crotalinae snake venom gland serine proteases. / Deshimaru, Masanobu; Ogawa, Tomohisa; Nakashima, Kinichi; Nobuhisa, Ikuo; Chijiwa, Takahito; Shimohigashi, Yasuyuki; Fukumaki, Yasuyuki; Niwa, Mineo; Yamashina, Ikuo; Hattori, Shosaku; Ohno, Motonori.

In: FEBS Letters, Vol. 397, No. 1, 11.11.1996, p. 83-88.

Research output: Contribution to journalArticle

Deshimaru, M, Ogawa, T, Nakashima, K, Nobuhisa, I, Chijiwa, T, Shimohigashi, Y, Fukumaki, Y, Niwa, M, Yamashina, I, Hattori, S & Ohno, M 1996, 'Accelerated evolution of crotalinae snake venom gland serine proteases', FEBS Letters, vol. 397, no. 1, pp. 83-88. https://doi.org/10.1016/S0014-5793(96)01144-1
Deshimaru M, Ogawa T, Nakashima K, Nobuhisa I, Chijiwa T, Shimohigashi Y et al. Accelerated evolution of crotalinae snake venom gland serine proteases. FEBS Letters. 1996 Nov 11;397(1):83-88. https://doi.org/10.1016/S0014-5793(96)01144-1
Deshimaru, Masanobu ; Ogawa, Tomohisa ; Nakashima, Kinichi ; Nobuhisa, Ikuo ; Chijiwa, Takahito ; Shimohigashi, Yasuyuki ; Fukumaki, Yasuyuki ; Niwa, Mineo ; Yamashina, Ikuo ; Hattori, Shosaku ; Ohno, Motonori. / Accelerated evolution of crotalinae snake venom gland serine proteases. In: FEBS Letters. 1996 ; Vol. 397, No. 1. pp. 83-88.
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