Accelerated evolution of crotalinae snake venom gland serine proteases

Masanobu Deshimaru; Tomohisa Ogawa; Kin ichi Nakashima; Ikuo Nobuhisa; Takahito Chijiwa; Yasuyuki Shimohigashi; Yasuyuki Fukumaki; Mineo Niwa; Ikuo Yamashina; Shosaku Hattori; Motonori Ohno

doi:10.1016/S0014-5793(96)01144-1

Accelerated evolution of crotalinae snake venom gland serine proteases

Masanobu Deshimaru, Tomohisa Ogawa, Kin ichi Nakashima, Ikuo Nobuhisa, Takahito Chijiwa, Yasuyuki Shimohigashi, Yasuyuki Fukumaki, Mineo Niwa, Ikuo Yamashina, Shosaku Hattori, Motonori Ohno

Stem Cell Biology and Medicine

Research output: Contribution to journal › Article › peer-review

156 Citations (Scopus)

Abstract

Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A₂ (PLA₂) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.

Original language	English
Pages (from-to)	83-88
Number of pages	6
Journal	FEBS Letters
Volume	397
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(96)01144-1
Publication status	Published - Nov 11 1996

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(96)01144-1

Cite this

@article{098745732f7142a4bc3583d34b057d19,

title = "Accelerated evolution of crotalinae snake venom gland serine proteases",

abstract = "Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A2 (PLA2) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.",

author = "Masanobu Deshimaru and Tomohisa Ogawa and Nakashima, {Kin ichi} and Ikuo Nobuhisa and Takahito Chijiwa and Yasuyuki Shimohigashi and Yasuyuki Fukumaki and Mineo Niwa and Ikuo Yamashina and Shosaku Hattori and Motonori Ohno",

year = "1996",

month = nov,

day = "11",

doi = "10.1016/S0014-5793(96)01144-1",

language = "English",

volume = "397",

pages = "83--88",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Accelerated evolution of crotalinae snake venom gland serine proteases

AU - Deshimaru, Masanobu

AU - Ogawa, Tomohisa

AU - Nakashima, Kin ichi

AU - Nobuhisa, Ikuo

AU - Chijiwa, Takahito

AU - Shimohigashi, Yasuyuki

AU - Fukumaki, Yasuyuki

AU - Niwa, Mineo

AU - Yamashina, Ikuo

AU - Hattori, Shosaku

AU - Ohno, Motonori

PY - 1996/11/11

Y1 - 1996/11/11

N2 - Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A2 (PLA2) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.

AB - Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A2 (PLA2) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.

UR - http://www.scopus.com/inward/record.url?scp=0030580350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030580350&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(96)01144-1

DO - 10.1016/S0014-5793(96)01144-1

M3 - Article

C2 - 8941719

AN - SCOPUS:0030580350

SN - 0014-5793

VL - 397

SP - 83

EP - 88

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Accelerated evolution of crotalinae snake venom gland serine proteases

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this