Accelerated evolution of Trimeresurus okinavensis venom gland phospholipase A2 isozyme-encoding genes

Ikuo Nobuhisa, Kin Ichi Nakashima, Masanobu Deshimaru, Tomohisa Ogawa, Yasuyuki Shimohigashi, Yasuyuki Fukumaki, Yoshiyuki Sakaki, Shosaku Hattori, Hiroshi Kihara, Motonori Ohno

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Three Trimeresurus okinavensis (To; himehabu snake, Crotalinae) venom gland phospholipase A2 (PLA2) isozyme-encoding genes, gPLA2-o1, gPLA2-o2 and gPLA2-o3, were isolated from its genomic DNA library. The nucleotide (nt) sequence analysis revealed that two of the three genes (gPLA2-o2 and gPLA2-o3) occasionally have been converted to inactivated genes by introduction of one base insertion or substitution. It was confirmed from Southern blot analysis that the To haploid genome contains only three venom gland PLA2 isozyme genes herein isolated. Comparison of these genes showed that nonsynonymous nt substitutions have occurred more frequently than synonymous nt substitutions in the protein-coding regions, except for the signal-peptide coding domain, implying that To venom gland PLA2 isozyme genes have evolved via accelerated evolution. Such an evolutionary feature of To venom gland PLA2 isozyme genes proves the general universality of accelerated evolution previously drawn for venom gland PLA2 isozyme genes of other crotalinae snakes. The variability in the mature protein-coding regions of three To venom gland PLA2 isozyme genes appears to have been brought about by natural selection for point mutations.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalGene
Volume172
Issue number2
DOIs
Publication statusPublished - Jun 26 1996

All Science Journal Classification (ASJC) codes

  • Genetics

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