Accelerated neutrophil apoptosis in mice lacking A1-a, a subtype of the bcl-2-related A1 gene

Azumi Hamasaki, Fujiro Sendo, Keiko Nakayama, Noriko Ishida, Izumi Negishi, Kei Ichi Nakayama, Shigetsugu Hatakeyama

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Abstract

To elucidate the role of A1, a new member of the Bcl-2 family of apoptosis regulators active in hematopoietic cell apoptosis, we established mice lacking A1-a, a subtype of the A1 gene in mice (Al-a(-/-) mice). Spontaneous apoptosis of peripheral blood neutrophils of A1-a(-/-) mice was enhanced compared with that of either wild-type mice or heterozygous mutants (A1-a(+/-) mice). Neutrophil apoptosis inhibition induced by lipopolysaccharide treatment in vitro or transendothelial migration in vivo observed in wild-type mice was abolished in both A1-a(-/-) and A1-a(+/-) animals. On the other hand, the extent of tumor necrosis factor α-induced acceleration of neutrophil apoptosis did not differ among A1-a(-/-), A1- a(+/-), and wild-type mice. The descending order oral mRNA expression was wild-type, A1-a(+/-), and A1-a(-/-). Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.

Original languageEnglish
Pages (from-to)1985-1992
Number of pages8
JournalJournal of Experimental Medicine
Volume188
Issue number11
DOIs
Publication statusPublished - Dec 7 1998

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Neutrophils
Apoptosis
Genes
Transendothelial and Transepithelial Migration
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Accelerated neutrophil apoptosis in mice lacking A1-a, a subtype of the bcl-2-related A1 gene. / Hamasaki, Azumi; Sendo, Fujiro; Nakayama, Keiko; Ishida, Noriko; Negishi, Izumi; Nakayama, Kei Ichi; Hatakeyama, Shigetsugu.

In: Journal of Experimental Medicine, Vol. 188, No. 11, 07.12.1998, p. 1985-1992.

Research output: Contribution to journalArticle

Hamasaki, A, Sendo, F, Nakayama, K, Ishida, N, Negishi, I, Nakayama, KI & Hatakeyama, S 1998, 'Accelerated neutrophil apoptosis in mice lacking A1-a, a subtype of the bcl-2-related A1 gene', Journal of Experimental Medicine, vol. 188, no. 11, pp. 1985-1992. https://doi.org/10.1084/jem.188.11.1985
Hamasaki, Azumi ; Sendo, Fujiro ; Nakayama, Keiko ; Ishida, Noriko ; Negishi, Izumi ; Nakayama, Kei Ichi ; Hatakeyama, Shigetsugu. / Accelerated neutrophil apoptosis in mice lacking A1-a, a subtype of the bcl-2-related A1 gene. In: Journal of Experimental Medicine. 1998 ; Vol. 188, No. 11. pp. 1985-1992.
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