Accentuation by pertussis toxin of the 5-hydroxytryptamine-induced potentiation of ATP-evoked responses in rat pheochromocytoma cells

Schuichi Koizumi, Makoto Ikeda, Ken Nakazawa, Kaori Inoue, Kunisuke Nagamatsu, Akira Hasegawa, Kazuhide Inoue

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We previously demonstrated that 5-hydroxytryptamine (5-HT) enhances the cationic current activated by extracellular ATP in rat pheochromocytoma PC12 cells. We report here that pertussis toxin (PTX) modulates this 5-HT-dependent enhancement in these cells. 5-HT potentiated ATP-evoked intracellular Ca2+ concentration ([Ca]i) rise and dopamine release over a concentration range from 1 to 100 μM. When cells were pre-treated with PTX, this potentiation was accentuated. Pretreatment with PTX also accentuated the 5-HT dependent enhancement of the ATP-activated current. These results suggest that the enhancement by 5-HT of the ATP-evoked responses is negatively regulated by a mechanism mediated through PTX-sensitive GTP-binding protein.

Original languageEnglish
Pages (from-to)104-107
Number of pages4
JournalNeuroscience Letters
Volume183
Issue number1-2
DOIs
Publication statusPublished - Jan 2 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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