Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA

Akiko Iwaki, Toru Iwaki, James E. Goldman, Koji Ogomori, Jun Tateishi, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

αB-Crystallin is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease. To examine whether or not accumulation of αB-crystallin is due to any abnormality of the gene structures, we determined the sequence of the αB-crystallin gene in two cases of pathologically confirmed Alexander's disease. Direct sequencing of the promoter and coding regions of the αB-crystallin gene in patients revealed them to have a normal sequence. Northern blotting showed a single αB-crystallin mRNA species expressed in the Alexander's disease brain.

Original languageEnglish
Pages (from-to)89-92
Number of pages4
JournalNeuroscience Letters
Volume140
Issue number1
DOIs
Publication statusPublished - Jun 8 1992

Fingerprint

Alexander Disease
Crystallins
5' Flanking Region
DNA
Brain
alpha-Crystallin B Chain
Genes
Genetic Promoter Regions
Northern Blotting
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA. / Iwaki, Akiko; Iwaki, Toru; Goldman, James E.; Ogomori, Koji; Tateishi, Jun; Sakaki, Yoshiyuki.

In: Neuroscience Letters, Vol. 140, No. 1, 08.06.1992, p. 89-92.

Research output: Contribution to journalArticle

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