Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA

Akiko Iwaki; Toru Iwaki; James E. Goldman; Koji Ogomori; Jun Tateishi; Yoshiyuki Sakaki

doi:10.1016/0304-3940(92)90689-5

Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA

Akiko Iwaki, Toru Iwaki, James E. Goldman, Koji Ogomori, Jun Tateishi, Yoshiyuki Sakaki

Department of Basic Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

αB-Crystallin is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease. To examine whether or not accumulation of αB-crystallin is due to any abnormality of the gene structures, we determined the sequence of the αB-crystallin gene in two cases of pathologically confirmed Alexander's disease. Direct sequencing of the promoter and coding regions of the αB-crystallin gene in patients revealed them to have a normal sequence. Northern blotting showed a single αB-crystallin mRNA species expressed in the Alexander's disease brain.

Original language	English
Pages (from-to)	89-92
Number of pages	4
Journal	Neuroscience Letters
Volume	140
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(92)90689-5
Publication status	Published - Jun 8 1992

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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10.1016/0304-3940(92)90689-5

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@article{df961bcffcb74233bea8beab2a6a78df,

title = "Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA",

abstract = "αB-Crystallin is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease. To examine whether or not accumulation of αB-crystallin is due to any abnormality of the gene structures, we determined the sequence of the αB-crystallin gene in two cases of pathologically confirmed Alexander's disease. Direct sequencing of the promoter and coding regions of the αB-crystallin gene in patients revealed them to have a normal sequence. Northern blotting showed a single αB-crystallin mRNA species expressed in the Alexander's disease brain.",

author = "Akiko Iwaki and Toru Iwaki and Goldman, {James E.} and Koji Ogomori and Jun Tateishi and Yoshiyuki Sakaki",

note = "Funding Information: The authors thank Drs. Berkely Powell and Melvin Ball for the Alexander's disease CNS tissue. This study was supported by a Grant-in-Aid for General Scientific Research fi'om the Ministry of Education, Science and Culture of Japan, by a Grant for Aging and Health from Ministry of Health and Welfare of Japan (T.I.) and by NIH Grants EY09331 and a Javits Neuroscience Investigator Award, NS17125, USA (J.E.G.).",

year = "1992",

month = jun,

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doi = "10.1016/0304-3940(92)90689-5",

language = "English",

volume = "140",

pages = "89--92",

journal = "Neuroscience Letters",

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T1 - Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA

AU - Iwaki, Akiko

AU - Iwaki, Toru

AU - Goldman, James E.

AU - Ogomori, Koji

AU - Tateishi, Jun

AU - Sakaki, Yoshiyuki

N1 - Funding Information: The authors thank Drs. Berkely Powell and Melvin Ball for the Alexander's disease CNS tissue. This study was supported by a Grant-in-Aid for General Scientific Research fi'om the Ministry of Education, Science and Culture of Japan, by a Grant for Aging and Health from Ministry of Health and Welfare of Japan (T.I.) and by NIH Grants EY09331 and a Javits Neuroscience Investigator Award, NS17125, USA (J.E.G.).

PY - 1992/6/8

Y1 - 1992/6/8

N2 - αB-Crystallin is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease. To examine whether or not accumulation of αB-crystallin is due to any abnormality of the gene structures, we determined the sequence of the αB-crystallin gene in two cases of pathologically confirmed Alexander's disease. Direct sequencing of the promoter and coding regions of the αB-crystallin gene in patients revealed them to have a normal sequence. Northern blotting showed a single αB-crystallin mRNA species expressed in the Alexander's disease brain.

AB - αB-Crystallin is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease. To examine whether or not accumulation of αB-crystallin is due to any abnormality of the gene structures, we determined the sequence of the αB-crystallin gene in two cases of pathologically confirmed Alexander's disease. Direct sequencing of the promoter and coding regions of the αB-crystallin gene in patients revealed them to have a normal sequence. Northern blotting showed a single αB-crystallin mRNA species expressed in the Alexander's disease brain.

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ER -

Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA

Abstract

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