Accumulation of human T lymphotropic virus type I-infected T cells in the salivary glands of patients with human T lymphotropic virus type I- associated Sjogren's syndrome

Yukiko Ohyama, Seiji Nakamura, Hideo Hara, Masanori Shinohara, Masanori Sasaki, Akiko Ikebe-Hiroki, Takefumi Mouri, Shizuka Tsunawaki, Kihachiro Abe, Kanemitsu Shirasuna, Kikuo Nomoto

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Abstract

Objective. To clarify the involvement of human T lymphotropic virus type I (HTLV-I) in the pathogenesis of Sjogren's syndrome (SS). Methods. In HTLV- I-seropositive patients with SS, HTLV-I proviral DNA in the labial salivary glands (SG) was detected by polymerase chain reaction (PCR) amplification of the extracted cellular DNA, and the localization in the SG was examined by in situ PCR hybridization. Results. The cellular DNA extracted from the SG contained full HTLV-I proviral DNA, which was present in the nucleus of the infiltrating T cells, but not in either the SG epithelial cells or the acinar cells. Furthermore, the viral loads in the SG were ~8 times to 9 x 103 times higher than those in the peripheral blood mononuclear cells. Conclusion. Accumulation of HTLV-I-infected T cells in the SG suggests that HTLV-I likely causes the self-reactive T cells to proliferate, which, as a result, induces SS.

Original languageEnglish
Pages (from-to)1972-1978
Number of pages7
JournalArthritis and rheumatism
Volume41
Issue number11
DOIs
Publication statusPublished - Nov 17 1998

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Sjogren's Syndrome
Salivary Glands
Viruses
T-Lymphocytes
DNA
Polymerase Chain Reaction
Human T-lymphotropic virus 1
Acinar Cells
Lip
Viral Load
Blood Cells
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Accumulation of human T lymphotropic virus type I-infected T cells in the salivary glands of patients with human T lymphotropic virus type I- associated Sjogren's syndrome. / Ohyama, Yukiko; Nakamura, Seiji; Hara, Hideo; Shinohara, Masanori; Sasaki, Masanori; Ikebe-Hiroki, Akiko; Mouri, Takefumi; Tsunawaki, Shizuka; Abe, Kihachiro; Shirasuna, Kanemitsu; Nomoto, Kikuo.

In: Arthritis and rheumatism, Vol. 41, No. 11, 17.11.1998, p. 1972-1978.

Research output: Contribution to journalArticle

Ohyama, Yukiko ; Nakamura, Seiji ; Hara, Hideo ; Shinohara, Masanori ; Sasaki, Masanori ; Ikebe-Hiroki, Akiko ; Mouri, Takefumi ; Tsunawaki, Shizuka ; Abe, Kihachiro ; Shirasuna, Kanemitsu ; Nomoto, Kikuo. / Accumulation of human T lymphotropic virus type I-infected T cells in the salivary glands of patients with human T lymphotropic virus type I- associated Sjogren's syndrome. In: Arthritis and rheumatism. 1998 ; Vol. 41, No. 11. pp. 1972-1978.
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abstract = "Objective. To clarify the involvement of human T lymphotropic virus type I (HTLV-I) in the pathogenesis of Sjogren's syndrome (SS). Methods. In HTLV- I-seropositive patients with SS, HTLV-I proviral DNA in the labial salivary glands (SG) was detected by polymerase chain reaction (PCR) amplification of the extracted cellular DNA, and the localization in the SG was examined by in situ PCR hybridization. Results. The cellular DNA extracted from the SG contained full HTLV-I proviral DNA, which was present in the nucleus of the infiltrating T cells, but not in either the SG epithelial cells or the acinar cells. Furthermore, the viral loads in the SG were ~8 times to 9 x 103 times higher than those in the peripheral blood mononuclear cells. Conclusion. Accumulation of HTLV-I-infected T cells in the SG suggests that HTLV-I likely causes the self-reactive T cells to proliferate, which, as a result, induces SS.",
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AU - Hara, Hideo

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AU - Mouri, Takefumi

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AB - Objective. To clarify the involvement of human T lymphotropic virus type I (HTLV-I) in the pathogenesis of Sjogren's syndrome (SS). Methods. In HTLV- I-seropositive patients with SS, HTLV-I proviral DNA in the labial salivary glands (SG) was detected by polymerase chain reaction (PCR) amplification of the extracted cellular DNA, and the localization in the SG was examined by in situ PCR hybridization. Results. The cellular DNA extracted from the SG contained full HTLV-I proviral DNA, which was present in the nucleus of the infiltrating T cells, but not in either the SG epithelial cells or the acinar cells. Furthermore, the viral loads in the SG were ~8 times to 9 x 103 times higher than those in the peripheral blood mononuclear cells. Conclusion. Accumulation of HTLV-I-infected T cells in the SG suggests that HTLV-I likely causes the self-reactive T cells to proliferate, which, as a result, induces SS.

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