Accumulation of p60lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171

Yoichi Moroi, Yasuhiro Koga, Kazuhiko Nakamura, Masumi Ohtsu, Genki Kimura, Kikuo Nomoto

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21 Citations (Scopus)

Abstract

The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56lck, whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti‐p56lck monoclonal antibody, was produced by using a 25‐amino‐acid synthetic polypeptide as the antigen, its structure corresponding to the N‐terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60lck, and the decrease of p56lck in human T cell leukemia virus type I (HTLV‐I)‐transformed T cell lines. Another anti‐Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C‐terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60lck in those HTLV‐1‐transformed T cell lines. The appearance of p60lck with the decrease of p56lck in normal T lymphocytes after stimulation suggested the origin of p60lck in HTLV‐I‐transformed T cells.

Original languageEnglish
Pages (from-to)909-915
Number of pages7
JournalJapanese Journal of Cancer Research
Volume82
Issue number8
DOIs
Publication statusPublished - Aug 1991

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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