Accumulation of p60 lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171

Yoichi Moroi, Yasuhiro Koga, Kazuhiko Nakamura, Masumi Ohtsu, Genki Kimura, Kikuo Nomoto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56 lck , whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti‐p56 lck monoclonal antibody, was produced by using a 25‐amino‐acid synthetic polypeptide as the antigen, its structure corresponding to the N‐terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60 lck , and the decrease of p56 lck in human T cell leukemia virus type I (HTLV‐I)‐transformed T cell lines. Another anti‐Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C‐terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60 lck in those HTLV‐1‐transformed T cell lines. The appearance of p60 lck with the decrease of p56 lck in normal T lymphocytes after stimulation suggested the origin of p60 lck in HTLV‐I‐transformed T cells.

Original languageEnglish
Pages (from-to)909-915
Number of pages7
JournalJapanese Journal of Cancer Research
Volume82
Issue number8
DOIs
Publication statusPublished - Jan 1 1991

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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Monoclonal Antibodies
T-Lymphocytes
Cell Line
Complementary DNA
Peptides
Transformed Cell Line
Human T-lymphotropic virus 1
Lymphocyte Activation
Antigens
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Accumulation of p60 lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171 . / Moroi, Yoichi; Koga, Yasuhiro; Nakamura, Kazuhiko; Ohtsu, Masumi; Kimura, Genki; Nomoto, Kikuo.

In: Japanese Journal of Cancer Research, Vol. 82, No. 8, 01.01.1991, p. 909-915.

Research output: Contribution to journalArticle

Moroi, Yoichi ; Koga, Yasuhiro ; Nakamura, Kazuhiko ; Ohtsu, Masumi ; Kimura, Genki ; Nomoto, Kikuo. / Accumulation of p60 lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171 In: Japanese Journal of Cancer Research. 1991 ; Vol. 82, No. 8. pp. 909-915.
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AU - Kimura, Genki

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