Acetaldehyde administrationinduces salsolinol formation in vivo in the dorsal striatum of Aldh2-knockout and C57BL/6N mice

Asuka Ito, Mostofa Jamal, Kiyoshi Ameno, Naoko Tanaka, Ayaka Takakura, Toshihiro Kawamoto, Kyoko Kitagawa, Keiichi Nakayama, Akiko Matsumoto, Takanori Miki, Hiroshi Kinoshita

Research output: Contribution to journalArticle

Abstract

Acetaldehyde (AcH) and salsolinol play important roles in the central effects of ethanol. This study aimed to investigate the effect of administration of AcH on dopamine (DA), DA-derived salsolinol and serotonin (5-HT) levels in the dorsal striatum of Aldh2-knockout (Aldh2-KO) and C57BL/6 N (WT) mice. Animals were treated with AcH (50, 100 and 200 mg/kg) intraperitoneally and dialysate levels of DA, 5-HT and salsolinol were determined using in vivo microdialysis coupled with HPLC-ECD. Salsolinol was first detected at 20 min after AcH administration, and reached its peak concentration (WT mice: 0.29 ± 0.22 pg/μl; Aldh2-KO mice: 0.63 ± 0.17 pg/μl) at 25 min in the 200 mg/kg AcH group, before decreasing rapidly and reaching zero at approximately 55–80 min. Treatment with 100 and 200 mg/kg AcH increased levels of salsolinol in both WT and Aldh2-KO mice, with 200 mg/kg AcH inducing a higher level of salsolinol in Aldh2-KO mice than in WT mice. Treatment with 50 mg/kg AcH produced a small increase in salsolinol levels in Aldh2-KO mice, whereas no elevation of salsolinol was detected in WT mice. The increase in salsolinol formation was found to occur a dose-dependent manner in both genotypes. Administration of AcH and the subsequent changes in salsolinol concentrations did not change DA or 5-HT levels in either genotype. Our study suggests that AcH dose-dependently increases the formation of salsolinol in the dorsal striatum of mice, which provides further support for the role of AcH in salsolinol formation in the animal brain.

Original languageEnglish
Pages (from-to)50-54
Number of pages5
JournalNeuroscience Letters
Volume685
DOIs
Publication statusPublished - Oct 15 2018

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Acetaldehyde
Inbred C57BL Mouse
Knockout Mice
Dopamine
Serotonin
salsolinol
Genotype
Dialysis Solutions
Microdialysis
Ethanol
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Acetaldehyde administrationinduces salsolinol formation in vivo in the dorsal striatum of Aldh2-knockout and C57BL/6N mice. / Ito, Asuka; Jamal, Mostofa; Ameno, Kiyoshi; Tanaka, Naoko; Takakura, Ayaka; Kawamoto, Toshihiro; Kitagawa, Kyoko; Nakayama, Keiichi; Matsumoto, Akiko; Miki, Takanori; Kinoshita, Hiroshi.

In: Neuroscience Letters, Vol. 685, 15.10.2018, p. 50-54.

Research output: Contribution to journalArticle

Ito, A, Jamal, M, Ameno, K, Tanaka, N, Takakura, A, Kawamoto, T, Kitagawa, K, Nakayama, K, Matsumoto, A, Miki, T & Kinoshita, H 2018, 'Acetaldehyde administrationinduces salsolinol formation in vivo in the dorsal striatum of Aldh2-knockout and C57BL/6N mice' Neuroscience Letters, vol. 685, pp. 50-54. https://doi.org/10.1016/j.neulet.2018.07.032
Ito, Asuka ; Jamal, Mostofa ; Ameno, Kiyoshi ; Tanaka, Naoko ; Takakura, Ayaka ; Kawamoto, Toshihiro ; Kitagawa, Kyoko ; Nakayama, Keiichi ; Matsumoto, Akiko ; Miki, Takanori ; Kinoshita, Hiroshi. / Acetaldehyde administrationinduces salsolinol formation in vivo in the dorsal striatum of Aldh2-knockout and C57BL/6N mice. In: Neuroscience Letters. 2018 ; Vol. 685. pp. 50-54.
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abstract = "Acetaldehyde (AcH) and salsolinol play important roles in the central effects of ethanol. This study aimed to investigate the effect of administration of AcH on dopamine (DA), DA-derived salsolinol and serotonin (5-HT) levels in the dorsal striatum of Aldh2-knockout (Aldh2-KO) and C57BL/6 N (WT) mice. Animals were treated with AcH (50, 100 and 200 mg/kg) intraperitoneally and dialysate levels of DA, 5-HT and salsolinol were determined using in vivo microdialysis coupled with HPLC-ECD. Salsolinol was first detected at 20 min after AcH administration, and reached its peak concentration (WT mice: 0.29 ± 0.22 pg/μl; Aldh2-KO mice: 0.63 ± 0.17 pg/μl) at 25 min in the 200 mg/kg AcH group, before decreasing rapidly and reaching zero at approximately 55–80 min. Treatment with 100 and 200 mg/kg AcH increased levels of salsolinol in both WT and Aldh2-KO mice, with 200 mg/kg AcH inducing a higher level of salsolinol in Aldh2-KO mice than in WT mice. Treatment with 50 mg/kg AcH produced a small increase in salsolinol levels in Aldh2-KO mice, whereas no elevation of salsolinol was detected in WT mice. The increase in salsolinol formation was found to occur a dose-dependent manner in both genotypes. Administration of AcH and the subsequent changes in salsolinol concentrations did not change DA or 5-HT levels in either genotype. Our study suggests that AcH dose-dependently increases the formation of salsolinol in the dorsal striatum of mice, which provides further support for the role of AcH in salsolinol formation in the animal brain.",
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AU - Ito, Asuka

AU - Jamal, Mostofa

AU - Ameno, Kiyoshi

AU - Tanaka, Naoko

AU - Takakura, Ayaka

AU - Kawamoto, Toshihiro

AU - Kitagawa, Kyoko

AU - Nakayama, Keiichi

AU - Matsumoto, Akiko

AU - Miki, Takanori

AU - Kinoshita, Hiroshi

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