Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Pyruvate ferredoxin oxidoreductase from Citrobacter sp. S-77 (PFORS77) was purified in order to develop a method for acetyl-CoA production. Although the purified PFORS77showed high O2-sensitivity, the activity could be remarkably stabilized in anaerobic conditions. PFORS77was effectively immobilized on ceramic hydroxyapatite (PFORS77-HA) with an efficiency of more than 96%, however, after encapsulation of PFORS77-HA in alginate, the rate of catalytic acetyl-CoA production was highly reduced to 36% when compared to that of the free enzyme. However, the operational stability of the PFORS77-HA in alginate hydrogels was remarkable, retaining over 68% initial activity even after ten repeated cycles. The results suggested that the PFORS77-HA hydrogels have a high potential for biotechnological application.

Original languageEnglish
Pages (from-to)279-285
Number of pages7
JournalBioresource Technology
Volume227
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Pyruvate Synthase
Acetyl Coenzyme A
Hydrogels
alginate
Alginate
encapsulation
Durapatite
Hydroxyapatite
Encapsulation
ceramics
anoxic conditions
Enzymes
enzyme
alginic acid
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels. / Takenaka, Makoto; Yoon, Ki Suk; Matsumoto, Takahiro; Ogo, Seiji.

In: Bioresource Technology, Vol. 227, 01.01.2017, p. 279-285.

Research output: Contribution to journalArticle

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