Acquisition of HIV type 1 resistance by β-chemokine-producing CD4+ T cells

Naoyoshi Maeda, Yoshio Koyanagi, Naoko Misawa, Naoko Miyano-Kurosaki, Jun Ichi Kira, Naoki Yamamoto

Research output: Contribution to journalArticle

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Abstract

The CD4+ T cell is a major target cell type for human immunodeficiency virus type 1 (HIV-1) infection, in this study, we provide evidence that the susceptibility to HIV-1 infection is variable in individual CD4+ T cells. Five CD4+ T cell clones were isolated from an HIV-1-seronegative donor and were investigated for their susceptibility to HIV-1 infection. Four CD4+ T cell clones were resistant to infection by a macrophage-tropic (R5) HIV-1 isolate whereas one clone was fully permissive. The level of susceptibility to HIV-1 correlated inversely with β-chemokine production, including RANTES (regulated on activation, normally T cell expressed and secreted), macrophage inflammatory protein 1α (MIP-1α), and MIP-1β. Resistance to HIV-1 infection was abrogated by the combined use of neutralizing antibodies against these three β-chemokines. Interestingly, a complete inhibition of HIV-1 infection was observed in peripheral blood mononuclear cells on infection induced by adding the culture supernatant or a small number of HIV, 1-resistant cell clones. Our results suggest the presence of a clonal self- defense mechanism within the CD4+ T cell population in vivo that involves the secretion of β-chemokines.

Original languageEnglish
Pages (from-to)1453-1460
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume15
Issue number16
DOIs
Publication statusPublished - Nov 1 1999

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Chemokines
HIV-1
T-Lymphocytes
Virus Diseases
Clone Cells
Macrophage Inflammatory Proteins
Infection
Neutralizing Antibodies
Blood Cells
Macrophages
Population

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Acquisition of HIV type 1 resistance by β-chemokine-producing CD4+ T cells. / Maeda, Naoyoshi; Koyanagi, Yoshio; Misawa, Naoko; Miyano-Kurosaki, Naoko; Kira, Jun Ichi; Yamamoto, Naoki.

In: AIDS Research and Human Retroviruses, Vol. 15, No. 16, 01.11.1999, p. 1453-1460.

Research output: Contribution to journalArticle

Maeda, N, Koyanagi, Y, Misawa, N, Miyano-Kurosaki, N, Kira, JI & Yamamoto, N 1999, 'Acquisition of HIV type 1 resistance by β-chemokine-producing CD4+ T cells', AIDS Research and Human Retroviruses, vol. 15, no. 16, pp. 1453-1460. https://doi.org/10.1089/088922299309964
Maeda, Naoyoshi ; Koyanagi, Yoshio ; Misawa, Naoko ; Miyano-Kurosaki, Naoko ; Kira, Jun Ichi ; Yamamoto, Naoki. / Acquisition of HIV type 1 resistance by β-chemokine-producing CD4+ T cells. In: AIDS Research and Human Retroviruses. 1999 ; Vol. 15, No. 16. pp. 1453-1460.
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