Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation

Atsushi Satomura, Yoshiaki Katsuyama, Natsuko Miura, Kouichi Kuroda, Ayako Tomio, Takeshi Bamba, Eiichiro Fukusaki, Mitsuyoshi Ueda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40°C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30°C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60-1. Furthermore, nontargeting metabolome analysis showed that YK60-1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8-1. In conclusion, S. cerevisiae MT8-1 acquired thermotolerance by induction of specific stress-responsive genes and enhanced intracellular trehalose levels.

Original languageEnglish
Pages (from-to)1116-1123
Number of pages8
JournalBiotechnology Progress
Volume29
Issue number5
DOIs
Publication statusPublished - Sep 1 2013
Externally publishedYes

Fingerprint

Trehalose
Breeding
Saccharomyces cerevisiae
Yeasts
Metabolome
Microarray Analysis
Heat-Shock Proteins
Oligonucleotide Array Sequence Analysis
Genes
Temperature
Enzymes
Growth
Thermotolerance

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation. / Satomura, Atsushi; Katsuyama, Yoshiaki; Miura, Natsuko; Kuroda, Kouichi; Tomio, Ayako; Bamba, Takeshi; Fukusaki, Eiichiro; Ueda, Mitsuyoshi.

In: Biotechnology Progress, Vol. 29, No. 5, 01.09.2013, p. 1116-1123.

Research output: Contribution to journalArticle

Satomura, A, Katsuyama, Y, Miura, N, Kuroda, K, Tomio, A, Bamba, T, Fukusaki, E & Ueda, M 2013, 'Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation', Biotechnology Progress, vol. 29, no. 5, pp. 1116-1123. https://doi.org/10.1002/btpr.1754
Satomura, Atsushi ; Katsuyama, Yoshiaki ; Miura, Natsuko ; Kuroda, Kouichi ; Tomio, Ayako ; Bamba, Takeshi ; Fukusaki, Eiichiro ; Ueda, Mitsuyoshi. / Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation. In: Biotechnology Progress. 2013 ; Vol. 29, No. 5. pp. 1116-1123.
@article{09078f65bc1749568c0fd0440c641e68,
title = "Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation",
abstract = "A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40°C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30°C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60-1. Furthermore, nontargeting metabolome analysis showed that YK60-1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8-1. In conclusion, S. cerevisiae MT8-1 acquired thermotolerance by induction of specific stress-responsive genes and enhanced intracellular trehalose levels.",
author = "Atsushi Satomura and Yoshiaki Katsuyama and Natsuko Miura and Kouichi Kuroda and Ayako Tomio and Takeshi Bamba and Eiichiro Fukusaki and Mitsuyoshi Ueda",
year = "2013",
month = "9",
day = "1",
doi = "10.1002/btpr.1754",
language = "English",
volume = "29",
pages = "1116--1123",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation

AU - Satomura, Atsushi

AU - Katsuyama, Yoshiaki

AU - Miura, Natsuko

AU - Kuroda, Kouichi

AU - Tomio, Ayako

AU - Bamba, Takeshi

AU - Fukusaki, Eiichiro

AU - Ueda, Mitsuyoshi

PY - 2013/9/1

Y1 - 2013/9/1

N2 - A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40°C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30°C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60-1. Furthermore, nontargeting metabolome analysis showed that YK60-1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8-1. In conclusion, S. cerevisiae MT8-1 acquired thermotolerance by induction of specific stress-responsive genes and enhanced intracellular trehalose levels.

AB - A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40°C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30°C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60-1. Furthermore, nontargeting metabolome analysis showed that YK60-1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8-1. In conclusion, S. cerevisiae MT8-1 acquired thermotolerance by induction of specific stress-responsive genes and enhanced intracellular trehalose levels.

UR - http://www.scopus.com/inward/record.url?scp=84886751118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886751118&partnerID=8YFLogxK

U2 - 10.1002/btpr.1754

DO - 10.1002/btpr.1754

M3 - Article

C2 - 24115578

AN - SCOPUS:84886751118

VL - 29

SP - 1116

EP - 1123

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 5

ER -