Actin filaments regulate microtubule growth at the centrosome

Daisuke Inoue, Dorian Obino, Judith Pineau, Francesca Farina, Jérémie Gaillard, Christophe Guerin, Laurent Blanchoin, Ana Maria Lennon-Duménil, Manuel Théry

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Abstract

The centrosome is the main microtubule-organizing centre. It also organizes a local network of actin filaments. However, the precise function of the actin network at the centrosome is not well understood. Here, we show that increasing densities of actin filaments at the centrosome of lymphocytes are correlated with reduced amounts of microtubules. Furthermore, lymphocyte activation resulted in disassembly of centrosomal actin and an increase in microtubule number. To further investigate the direct crosstalk between actin and microtubules at the centrosome, we performed in vitro reconstitution assays based on (i) purified centrosomes and (ii) on the co-micropatterning of microtubule seeds and actin filaments. These two assays demonstrated that actin filaments constitute a physical barrier blocking elongation of nascent microtubules. Finally, we showed that cell adhesion and cell spreading lead to lower densities of centrosomal actin, thus resulting in higher microtubule growth. We therefore propose a novel mechanism, by which the number of centrosomal microtubules is regulated by cell adhesion and actin-network architecture.

Original languageEnglish
Article numbere99630
JournalEMBO Journal
Volume38
Issue number11
DOIs
Publication statusPublished - Jun 3 2019
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Inoue, D., Obino, D., Pineau, J., Farina, F., Gaillard, J., Guerin, C., ... Théry, M. (2019). Actin filaments regulate microtubule growth at the centrosome. EMBO Journal, 38(11), [e99630]. https://doi.org/10.15252/embj.201899630