Action of hormone responsive sequence in 2.3 kb promoter of CYP11A1

Yu Yao Huang, Meng Chun Hu, Nai Chi Hsu, Chi Kuang Leo Wang, Bon Chu Chung

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Abstract

The CYP11A1 gene encodes cytochrome P450scc, the enzyme catalyzing the first step of steroid biosynthesis in the adrenal and gonad. We generated transgenic mice containing 2.3 kb of the 5′-flanking region of CYP11A1 driving LacZ reporter gene expression, in order to study hormonal control of CYP11A1 gene expression in different tissues. This 2.3 kb fragment contains information for hormonal control; by ACTH and hCG which increased reporter gene expression, in the adrenal and testis of transgenic mice respectively, while dexamethasone administration decreased reporter activity in the adrenal. The 5′-fragment of CYP11A1 has appreciable promoter activities in mouse adrenal Y1 cells but not in non-steroidogenic COS-1 cells, showing cell-type specificity. Transcription factor SF-1 activates the 2.3 kb promoter, which can be potentiated by cotransfection with c-Jun in steroidogenic JEG3 cells but not in COS-1 cells. We conclude that the 2.3 kb region of CYP11A1 contains elements controlling hormonal-dependent, cell-type-specific expression. In addition, c-Jun and SF-1 could act synergistically to activate CYP11A1 gene expression.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume175
Issue number1-2
DOIs
Publication statusPublished - Apr 25 2001

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Huang, Y. Y., Hu, M. C., Hsu, N. C., Wang, C. K. L., & Chung, B. C. (2001). Action of hormone responsive sequence in 2.3 kb promoter of CYP11A1. Molecular and Cellular Endocrinology, 175(1-2), 205-210. https://doi.org/10.1016/S0303-7207(01)00388-4