Activated and mature CD83-positive dendritic cells and interferon-γ-positive cells in skin eruptions of secondary syphilis

Tetsuya Koga, Hong Duan, Yoichi Moroi, Kazunori Urabe, Masutaka Furue

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Dendritic cells are considered to be the most potent antigen-presenting cells, and CD83 is expressed at a high level in immunocompetent, activated and mature dendritic cells. Various pathogens can activate and modulate the function of dendritic cells. The presence of activated and mature dendritic cells in skin lesions of secondary syphilis has never been reported. In the present study, an immunohistochemical technique was used to determine the exact tissue distributions of CD83+ dendritic cells and interferon-γ+ cells in skin lesions of patients with secondary syphilis. Immunohistochemical staining was performed by using formalin-fixed, paraffin-embedded sections. A small but significant subpopulation of CD83+ dendritic cells was found in the dermis. CD83+ dendritic cells were in close contact with lymphocytes. High-intensity staining of CD83 antigens was detected not only on the surface but also in the cytoplasm of dendritic cells. Infiltrating mononuclear cells were stained positively for CD4 or CD8, with CD8+ cells always being in the majority. A small number of interferon-γ+ cells resembling mononuclear lymphoid cells were detected in all samples. These results provide in vivo support for the hypothesis that dendritic cells are activated by Treponema pallidum and that thus activated and mature CD83+ dendritic cells may play a role in the Th1 response in secondary syphilis.

Original languageEnglish
Pages (from-to)214-217
Number of pages4
JournalActa Dermato-Venereologica
Volume83
Issue number3
DOIs
Publication statusPublished - Jun 9 2003

Fingerprint

Dendritic Cells
Interferons
Skin
Lymphocytes
Staining and Labeling
Treponema pallidum
Secondary syphilis
Langerhans Cells
Antigen-Presenting Cells
Tissue Distribution
Dermis
Paraffin
Formaldehyde
Cytoplasm
Cell Count

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Activated and mature CD83-positive dendritic cells and interferon-γ-positive cells in skin eruptions of secondary syphilis. / Koga, Tetsuya; Duan, Hong; Moroi, Yoichi; Urabe, Kazunori; Furue, Masutaka.

In: Acta Dermato-Venereologica, Vol. 83, No. 3, 09.06.2003, p. 214-217.

Research output: Contribution to journalArticle

@article{54c9dc282ed84b6ba330ee794353e1de,
title = "Activated and mature CD83-positive dendritic cells and interferon-γ-positive cells in skin eruptions of secondary syphilis",
abstract = "Dendritic cells are considered to be the most potent antigen-presenting cells, and CD83 is expressed at a high level in immunocompetent, activated and mature dendritic cells. Various pathogens can activate and modulate the function of dendritic cells. The presence of activated and mature dendritic cells in skin lesions of secondary syphilis has never been reported. In the present study, an immunohistochemical technique was used to determine the exact tissue distributions of CD83+ dendritic cells and interferon-γ+ cells in skin lesions of patients with secondary syphilis. Immunohistochemical staining was performed by using formalin-fixed, paraffin-embedded sections. A small but significant subpopulation of CD83+ dendritic cells was found in the dermis. CD83+ dendritic cells were in close contact with lymphocytes. High-intensity staining of CD83 antigens was detected not only on the surface but also in the cytoplasm of dendritic cells. Infiltrating mononuclear cells were stained positively for CD4 or CD8, with CD8+ cells always being in the majority. A small number of interferon-γ+ cells resembling mononuclear lymphoid cells were detected in all samples. These results provide in vivo support for the hypothesis that dendritic cells are activated by Treponema pallidum and that thus activated and mature CD83+ dendritic cells may play a role in the Th1 response in secondary syphilis.",
author = "Tetsuya Koga and Hong Duan and Yoichi Moroi and Kazunori Urabe and Masutaka Furue",
year = "2003",
month = "6",
day = "9",
doi = "10.1080/00015550310007247",
language = "English",
volume = "83",
pages = "214--217",
journal = "Acta Dermato-Venereologica",
issn = "0001-5555",
publisher = "Society for the Publication of Acta Dermato-Venereologica",
number = "3",

}

TY - JOUR

T1 - Activated and mature CD83-positive dendritic cells and interferon-γ-positive cells in skin eruptions of secondary syphilis

AU - Koga, Tetsuya

AU - Duan, Hong

AU - Moroi, Yoichi

AU - Urabe, Kazunori

AU - Furue, Masutaka

PY - 2003/6/9

Y1 - 2003/6/9

N2 - Dendritic cells are considered to be the most potent antigen-presenting cells, and CD83 is expressed at a high level in immunocompetent, activated and mature dendritic cells. Various pathogens can activate and modulate the function of dendritic cells. The presence of activated and mature dendritic cells in skin lesions of secondary syphilis has never been reported. In the present study, an immunohistochemical technique was used to determine the exact tissue distributions of CD83+ dendritic cells and interferon-γ+ cells in skin lesions of patients with secondary syphilis. Immunohistochemical staining was performed by using formalin-fixed, paraffin-embedded sections. A small but significant subpopulation of CD83+ dendritic cells was found in the dermis. CD83+ dendritic cells were in close contact with lymphocytes. High-intensity staining of CD83 antigens was detected not only on the surface but also in the cytoplasm of dendritic cells. Infiltrating mononuclear cells were stained positively for CD4 or CD8, with CD8+ cells always being in the majority. A small number of interferon-γ+ cells resembling mononuclear lymphoid cells were detected in all samples. These results provide in vivo support for the hypothesis that dendritic cells are activated by Treponema pallidum and that thus activated and mature CD83+ dendritic cells may play a role in the Th1 response in secondary syphilis.

AB - Dendritic cells are considered to be the most potent antigen-presenting cells, and CD83 is expressed at a high level in immunocompetent, activated and mature dendritic cells. Various pathogens can activate and modulate the function of dendritic cells. The presence of activated and mature dendritic cells in skin lesions of secondary syphilis has never been reported. In the present study, an immunohistochemical technique was used to determine the exact tissue distributions of CD83+ dendritic cells and interferon-γ+ cells in skin lesions of patients with secondary syphilis. Immunohistochemical staining was performed by using formalin-fixed, paraffin-embedded sections. A small but significant subpopulation of CD83+ dendritic cells was found in the dermis. CD83+ dendritic cells were in close contact with lymphocytes. High-intensity staining of CD83 antigens was detected not only on the surface but also in the cytoplasm of dendritic cells. Infiltrating mononuclear cells were stained positively for CD4 or CD8, with CD8+ cells always being in the majority. A small number of interferon-γ+ cells resembling mononuclear lymphoid cells were detected in all samples. These results provide in vivo support for the hypothesis that dendritic cells are activated by Treponema pallidum and that thus activated and mature CD83+ dendritic cells may play a role in the Th1 response in secondary syphilis.

UR - http://www.scopus.com/inward/record.url?scp=0038578300&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038578300&partnerID=8YFLogxK

U2 - 10.1080/00015550310007247

DO - 10.1080/00015550310007247

M3 - Article

VL - 83

SP - 214

EP - 217

JO - Acta Dermato-Venereologica

JF - Acta Dermato-Venereologica

SN - 0001-5555

IS - 3

ER -