Activation and polar sequestration of PopA, a c-di-GMP effector protein involved in Caulobacter crescentus cell cycle control

Shogo Ozaki, Annina Schalch-Moser, Ludwig Zumthor, Pablo Manfredi, Anna Ebbensgaard, Tilman Schirmer, Urs Jenal

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Summary: When Caulobacter crescentus enters S-phase the replication initiation inhibitor CtrA dynamically positions to the old cell pole to be degraded by the polar ClpXP protease. Polar delivery of CtrA requires PopA and the diguanylate cyclase PleD that positions to the same pole. Here we present evidence that PopA originated through gene duplication from its paralogue response regulator PleD and subsequent co-option as c-di-GMP effector protein. While the C-terminal catalytic domain (GGDEF) of PleD is activated by phosphorylation of the N-terminal receiver domain, functional adaptation has reversed signal transduction in PopA with the GGDEF domain adopting input function and the receiver domain serving as regulatory output. We show that the N-terminal receiver domain of PopA specifically interacts with RcdA, a component required for CtrA degradation. In contrast, the GGDEF domain serves to target PopA to the cell pole in response to c-di-GMP binding. In agreement with the divergent activation and targeting mechanisms, distinct markers sequester PleD and PopA to the old cell pole upon S-phase entry. Together these data indicate that PopA adopted a novel role as topology specificity factor to help recruit components of the CtrA degradation pathway to the protease specific old cell pole of C. crescentus.

Original languageEnglish
Pages (from-to)580-594
Number of pages15
JournalMolecular Microbiology
Volume94
Issue number3
DOIs
Publication statusPublished - Nov 1 2014

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Caulobacter crescentus
Cell Cycle Checkpoints
S Phase
Proteins
Peptide Hydrolases
Gene Duplication
Signal Transduction
Catalytic Domain
Phosphorylation
bis(3',5')-cyclic diguanylic acid

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Activation and polar sequestration of PopA, a c-di-GMP effector protein involved in Caulobacter crescentus cell cycle control. / Ozaki, Shogo; Schalch-Moser, Annina; Zumthor, Ludwig; Manfredi, Pablo; Ebbensgaard, Anna; Schirmer, Tilman; Jenal, Urs.

In: Molecular Microbiology, Vol. 94, No. 3, 01.11.2014, p. 580-594.

Research output: Contribution to journalArticle

Ozaki, Shogo ; Schalch-Moser, Annina ; Zumthor, Ludwig ; Manfredi, Pablo ; Ebbensgaard, Anna ; Schirmer, Tilman ; Jenal, Urs. / Activation and polar sequestration of PopA, a c-di-GMP effector protein involved in Caulobacter crescentus cell cycle control. In: Molecular Microbiology. 2014 ; Vol. 94, No. 3. pp. 580-594.
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