TY - JOUR
T1 - Activation of cultured bovine aortic endothelial cells by extracellular pyrimidine triphosphate
AU - Takanari, Kitazono
AU - Koichiro, Takeshige
AU - Shigeki, Minakami
PY - 1992/8
Y1 - 1992/8
N2 - 1. 1. The addition of ATP to cultured bovine aortic endothelial cells induced the increase in intracellular free calcium concentration ([Ca2+]i) and thereby activated the sodium/proton exchanger and the prostacyclin production in a similar dose-dependent manner, as observed by the addition of ATP. 2. 2. Other nucleoside triphosphates also activated the cells and the potency orders of the nucleotides were ATP > UTP > ITP > CTP > GTP for all the responses. 3. 3. Pretreatment of the cells with UTP desensitized the response to ATP and the pretreatment of ATP desensitized the response to UTP. 4. 4. The responses to ATP and UTP were inhibited by neither pertussis nor cholera toxin. 5. 5. The receptor for UTP, however, may be a distinct pyrimidinoceptor different from the purinoceptor of the cells for ATP, because the 50% effective concentration of UDP was much larger than that of UTP, while ATP and ADP were essentially equipotent ligands to the endothelial cells.
AB - 1. 1. The addition of ATP to cultured bovine aortic endothelial cells induced the increase in intracellular free calcium concentration ([Ca2+]i) and thereby activated the sodium/proton exchanger and the prostacyclin production in a similar dose-dependent manner, as observed by the addition of ATP. 2. 2. Other nucleoside triphosphates also activated the cells and the potency orders of the nucleotides were ATP > UTP > ITP > CTP > GTP for all the responses. 3. 3. Pretreatment of the cells with UTP desensitized the response to ATP and the pretreatment of ATP desensitized the response to UTP. 4. 4. The responses to ATP and UTP were inhibited by neither pertussis nor cholera toxin. 5. 5. The receptor for UTP, however, may be a distinct pyrimidinoceptor different from the purinoceptor of the cells for ATP, because the 50% effective concentration of UDP was much larger than that of UTP, while ATP and ADP were essentially equipotent ligands to the endothelial cells.
UR - http://www.scopus.com/inward/record.url?scp=0026625229&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026625229&partnerID=8YFLogxK
U2 - 10.1016/0020-711X(92)90208-I
DO - 10.1016/0020-711X(92)90208-I
M3 - Article
C2 - 1644213
AN - SCOPUS:0026625229
VL - 24
SP - 1323
EP - 1327
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
SN - 1357-2725
IS - 8
ER -