Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide

Koei Kawakami; Taiki Urakawa; Yasuhiro Oda; Yoshio Iwai

doi:10.1002/jctb.2178

Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide

Koei Kawakami, Taiki Urakawa, Yasuhiro Oda, Yoshio Iwai

Molecular and Biochemical Systems Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Background: Sol-gel entrapment of lipases is usually performed in an aqueous solution. A novel method of sol-gel coating of lipasde in supercritical carbon dioxide (SC-CO₂) is proposed. Results: Crude lipase powder (Rhizopus oryzae) coatedwith hydrophobic silicates, derived from dimethyldimethoxysilane and tetramethoxysilane in SC-CO₂ at 35 °C and 15 MPa, exhibited 5-7 times higher esterification activity than that prepared via an aqueous sol-gel route. Lipase immobilized in amethyl-substituted silica monolithwas also highly activated by sol-gel coating using the same silica precursors in SC-CO₂. Conclusion: Sol-gel coating in SC-CO₂, of lipases in powder and immobilized forms with hydrophobic alkyl-substituted silicates provides an efficient tool for the enhancement of enzymatic activities in non-aqueous media.

Original language	English
Pages (from-to)	1412-1417
Number of pages	6
Journal	Journal of Chemical Technology and Biotechnology
Volume	84
Issue number	9
DOIs	https://doi.org/10.1002/jctb.2178
Publication status	Published - Oct 5 2009

All Science Journal Classification (ASJC) codes

Biotechnology
Chemical Engineering(all)
Renewable Energy, Sustainability and the Environment
Fuel Technology
Waste Management and Disposal
Pollution
Organic Chemistry
Inorganic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jctb.2178

Cite this

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title = "Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide",

abstract = "Background: Sol-gel entrapment of lipases is usually performed in an aqueous solution. A novel method of sol-gel coating of lipasde in supercritical carbon dioxide (SC-CO2) is proposed. Results: Crude lipase powder (Rhizopus oryzae) coatedwith hydrophobic silicates, derived from dimethyldimethoxysilane and tetramethoxysilane in SC-CO2 at 35 °C and 15 MPa, exhibited 5-7 times higher esterification activity than that prepared via an aqueous sol-gel route. Lipase immobilized in amethyl-substituted silica monolithwas also highly activated by sol-gel coating using the same silica precursors in SC-CO2. Conclusion: Sol-gel coating in SC-CO2, of lipases in powder and immobilized forms with hydrophobic alkyl-substituted silicates provides an efficient tool for the enhancement of enzymatic activities in non-aqueous media.",

author = "Koei Kawakami and Taiki Urakawa and Yasuhiro Oda and Yoshio Iwai",

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T1 - Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide

AU - Kawakami, Koei

AU - Urakawa, Taiki

AU - Oda, Yasuhiro

AU - Iwai, Yoshio

PY - 2009/10/5

Y1 - 2009/10/5

N2 - Background: Sol-gel entrapment of lipases is usually performed in an aqueous solution. A novel method of sol-gel coating of lipasde in supercritical carbon dioxide (SC-CO2) is proposed. Results: Crude lipase powder (Rhizopus oryzae) coatedwith hydrophobic silicates, derived from dimethyldimethoxysilane and tetramethoxysilane in SC-CO2 at 35 °C and 15 MPa, exhibited 5-7 times higher esterification activity than that prepared via an aqueous sol-gel route. Lipase immobilized in amethyl-substituted silica monolithwas also highly activated by sol-gel coating using the same silica precursors in SC-CO2. Conclusion: Sol-gel coating in SC-CO2, of lipases in powder and immobilized forms with hydrophobic alkyl-substituted silicates provides an efficient tool for the enhancement of enzymatic activities in non-aqueous media.

AB - Background: Sol-gel entrapment of lipases is usually performed in an aqueous solution. A novel method of sol-gel coating of lipasde in supercritical carbon dioxide (SC-CO2) is proposed. Results: Crude lipase powder (Rhizopus oryzae) coatedwith hydrophobic silicates, derived from dimethyldimethoxysilane and tetramethoxysilane in SC-CO2 at 35 °C and 15 MPa, exhibited 5-7 times higher esterification activity than that prepared via an aqueous sol-gel route. Lipase immobilized in amethyl-substituted silica monolithwas also highly activated by sol-gel coating using the same silica precursors in SC-CO2. Conclusion: Sol-gel coating in SC-CO2, of lipases in powder and immobilized forms with hydrophobic alkyl-substituted silicates provides an efficient tool for the enhancement of enzymatic activities in non-aqueous media.

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Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide

Abstract

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UN SDGs

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