Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis

Dae Hwi Park, Sung Jun Hong, Ryan D. Salinas, Siyuan John Liu, Shawn W. Sun, Jacopo Sgualdino, Giuseppe Testa, Martin M. Matzuk, Naoki Iwamori, Daniel A. Lim

Research output: Contribution to journalArticle

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Abstract

The epigenetic mechanisms that enable lifelong neurogenesis from neural stem cells (NSCs) in the adult mammalian brain are poorly understood. Here, we show that JMJD3, a histone H3 lysine 27 (H3K27) demethylase, acts as a critical activator of neurogenesis from adult subventricular zone (SVZ) NSCs. JMJD3 is upregulated in neuroblasts, and Jmjd3 deletion targeted to SVZ NSCs in both developing and adult mice impairs neuronal differentiation. JMJD3 regulates neurogenic gene expression via interaction at not only promoter regions but also neurogenic enhancer elements. JMJD3 localizes at neural enhancers genome-wide in embryonic brain, and in SVZ NSCs, JMJD3 regulates the I12b enhancer of Dlx2. In Jmjd3-deleted SVZ cells, I12b remains enriched with H3K27me3 and Dlx2-dependent neurogenesis fails. These findings support a model in which JMJD3 and the poised state of key transcriptional regulatory elements comprise an epigenetic mechanism that enables the activation of neurogenic gene expression in adult NSCs throughout life.

Original languageEnglish
Pages (from-to)1290-1299
Number of pages10
JournalCell Reports
Volume8
Issue number5
DOIs
Publication statusPublished - Jan 1 2014

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Neural Stem Cells
Neurogenesis
Stem cells
Gene expression
Lateral Ventricles
Brain
Chemical activation
Gene Expression
Epigenomics
Transcriptional Regulatory Elements
Adult Stem Cells
Genetic Promoter Regions
Histones
Lysine
Genes
Genome

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Park, D. H., Hong, S. J., Salinas, R. D., Liu, S. J., Sun, S. W., Sgualdino, J., ... Lim, D. A. (2014). Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis. Cell Reports, 8(5), 1290-1299. https://doi.org/10.1016/j.celrep.2014.07.060

Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis. / Park, Dae Hwi; Hong, Sung Jun; Salinas, Ryan D.; Liu, Siyuan John; Sun, Shawn W.; Sgualdino, Jacopo; Testa, Giuseppe; Matzuk, Martin M.; Iwamori, Naoki; Lim, Daniel A.

In: Cell Reports, Vol. 8, No. 5, 01.01.2014, p. 1290-1299.

Research output: Contribution to journalArticle

Park, DH, Hong, SJ, Salinas, RD, Liu, SJ, Sun, SW, Sgualdino, J, Testa, G, Matzuk, MM, Iwamori, N & Lim, DA 2014, 'Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis', Cell Reports, vol. 8, no. 5, pp. 1290-1299. https://doi.org/10.1016/j.celrep.2014.07.060
Park, Dae Hwi ; Hong, Sung Jun ; Salinas, Ryan D. ; Liu, Siyuan John ; Sun, Shawn W. ; Sgualdino, Jacopo ; Testa, Giuseppe ; Matzuk, Martin M. ; Iwamori, Naoki ; Lim, Daniel A. / Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis. In: Cell Reports. 2014 ; Vol. 8, No. 5. pp. 1290-1299.
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