Activation of NF-κB promotes the transition of large, CD43+ pre-B cells to small, CD43- pre-B cells

Eijiro Jimi, Roderick J. Phillips, Mercedes Rincon, Reinhard Voll, Hajime Karasuyama, Richard Flavell, Sankar Ghosh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The regulation of the transcription factor nuclear factor-κB (NF-κB) during B-cell development was examined using cells isolated from the bone marrow of transgenic mice expressing a κB luciferase reporter gene. The results indicate that the highest level of NF-κB activity is present in cells expressing the pre-B-cell receptor. Furthermore, cross-linking of Igβ on CD43+ pre-B cells is able to activate NF-κB in recombination-activating gene 1-deficient mice, preceding their further differentiation into CD43- pre-B cells. Expression of a dominant negative form of IκBα using a transgenic approach or by retroviral infection leads to a reduction in the number of CD43+ pre-B cells. These data therefore indicate that activation of NF-κB in CD43+ pre-B cells, as a result of signaling by the pre-B-cell receptor, facilitates the continued development of large, CD43+ pre-B cells into small CD43- pre-B cells.

Original languageEnglish
Pages (from-to)815-825
Number of pages11
JournalInternational Immunology
Volume17
Issue number6
DOIs
Publication statusPublished - Jun 1 2005
Externally publishedYes

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B-Lymphoid Precursor Cells
Pre-B Cell Receptors
RAG-1 Genes
Luciferases
Reporter Genes
Bone Marrow Cells
Transgenic Mice
B-Lymphocytes
Transcription Factors
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Activation of NF-κB promotes the transition of large, CD43+ pre-B cells to small, CD43- pre-B cells. / Jimi, Eijiro; Phillips, Roderick J.; Rincon, Mercedes; Voll, Reinhard; Karasuyama, Hajime; Flavell, Richard; Ghosh, Sankar.

In: International Immunology, Vol. 17, No. 6, 01.06.2005, p. 815-825.

Research output: Contribution to journalArticle

Jimi, E, Phillips, RJ, Rincon, M, Voll, R, Karasuyama, H, Flavell, R & Ghosh, S 2005, 'Activation of NF-κB promotes the transition of large, CD43+ pre-B cells to small, CD43- pre-B cells', International Immunology, vol. 17, no. 6, pp. 815-825. https://doi.org/10.1093/intimm/dxh263
Jimi, Eijiro ; Phillips, Roderick J. ; Rincon, Mercedes ; Voll, Reinhard ; Karasuyama, Hajime ; Flavell, Richard ; Ghosh, Sankar. / Activation of NF-κB promotes the transition of large, CD43+ pre-B cells to small, CD43- pre-B cells. In: International Immunology. 2005 ; Vol. 17, No. 6. pp. 815-825.
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