Activation of T cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis

Shigehisa Tanaka, Yuki Kimura, Akio Mitani, Genta Yamamoto, Hitoshi Nishimura, Ralf Spallek, Mahavir Singh, Toshihide Noguchi, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    71 Citations (Scopus)

    Abstract

    We have previously reported that CD4 + T cells recognizing a peptide comprising residues 234-252 of the heat shock protein (HSP)70 of Mycobacterium tuberculosis (M.tb) in the context of RT1.B MHC class H molecule emerged in the peritoneal cavity during the course of Listeria monocytogenes infection in rats and suppressed the inflammatory responses against listerial infection via IL-10 production. We report in this work that pretreatment with peptide 234-252 of HSP70 derived from M.tb suppressed the development of adjuvant arthritis (AA) in Lewis rats induced using heat- killed M.tb. T cells from rats pretreated with peptide 234-252 produced a significant amount of IL-10 in response to the epitope. T cells from rats pretreated with the peptide and immunized with M.tb produced the larger amount of IL-10 in response to the peptide, but only a marginal level of IFN- γ in response to purified protein derivative of M.tb. Administration of anti-IL-10 Ab partly inhibited the suppressive effect of pretreatment with peptide 234-252 on the development of AA. Furthermore, transfer of a T cell line specific for the epitope at the time of AA induction markedly suppressed AA. These findings suggested that T cells recognizing peptide 234-252 may play a regulatory role in inflammation during AA via the production of suppressive cytokines including IL-10.

    Original languageEnglish
    Pages (from-to)5560-5565
    Number of pages6
    JournalJournal of Immunology
    Volume163
    Issue number10
    Publication statusPublished - Nov 15 1999

    Fingerprint

    HSP70 Heat-Shock Proteins
    T-Lymphocyte Epitopes
    Experimental Arthritis
    Mycobacterium tuberculosis
    Interleukin-10
    Peptides
    T-Lymphocytes
    Epitopes
    Listeriosis
    Peritoneal Cavity
    Listeria monocytogenes
    Hot Temperature
    Cytokines
    Inflammation
    Cell Line
    Infection

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Tanaka, S., Kimura, Y., Mitani, A., Yamamoto, G., Nishimura, H., Spallek, R., ... Yoshikai, Y. (1999). Activation of T cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis. Journal of Immunology, 163(10), 5560-5565.

    Activation of T cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis. / Tanaka, Shigehisa; Kimura, Yuki; Mitani, Akio; Yamamoto, Genta; Nishimura, Hitoshi; Spallek, Ralf; Singh, Mahavir; Noguchi, Toshihide; Yoshikai, Yasunobu.

    In: Journal of Immunology, Vol. 163, No. 10, 15.11.1999, p. 5560-5565.

    Research output: Contribution to journalArticle

    Tanaka, S, Kimura, Y, Mitani, A, Yamamoto, G, Nishimura, H, Spallek, R, Singh, M, Noguchi, T & Yoshikai, Y 1999, 'Activation of T cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis', Journal of Immunology, vol. 163, no. 10, pp. 5560-5565.
    Tanaka S, Kimura Y, Mitani A, Yamamoto G, Nishimura H, Spallek R et al. Activation of T cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis. Journal of Immunology. 1999 Nov 15;163(10):5560-5565.
    Tanaka, Shigehisa ; Kimura, Yuki ; Mitani, Akio ; Yamamoto, Genta ; Nishimura, Hitoshi ; Spallek, Ralf ; Singh, Mahavir ; Noguchi, Toshihide ; Yoshikai, Yasunobu. / Activation of T cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis. In: Journal of Immunology. 1999 ; Vol. 163, No. 10. pp. 5560-5565.
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