Activation of tongue-expressed GPR40 and GPR120 by non caloric agonists is not sufficient to drive preference in mice

N. Godinot, Keiko Nakano, M. E. Barcos, N. Pineau, M. Ledda, F. Viton, Y. Ninomiya, J. le Coutre, S. Damak

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    There is mounting evidence that, in addition to texture and olfaction, taste plays a role in the detection of long chain fatty acids. Triglycerides, the main components of oils and dietary fat, are hydrolyzed in the mouth by a lingual lipase secreted from the von Ebner gland and the released free fatty acids are detected by the taste system. GPR40 and GPR120, two fatty acid responsive G-protein-coupled receptors (GPCRs), are expressed in taste bud cells, and knockout mice lacking either of those receptors have blunted taste nerve responses to and reduced preference for fatty acids. Here we investigated whether activation of those GPCRs is sufficient to elicit fat taste and preference. Five non-fatty acid agonists of GPR40 and two non-fatty acid agonists of GPR120 activated the glossopharyngeal nerve of wild-type mice but not of knockout mice lacking the cognate receptor. In human subjects, two-alternative forced choice (2-AFC) tests, triangle tests and sensory profiling showed that non fatty acid agonists of GPR40 dissolved in water are detected in sip and spit tests and elicit a taste similar to that of linoleic acid, whereas 2-AFC tests showed that two agonists of GPR120 in water are not perceived fattier than water alone. Wild-type mice did not show any preference for five agonists of GPR40, two agonists of GPR120 and mixtures of both agonists over water in two-bottle preference tests. Together these data indicate that GPR40 mediated taste perception is not sufficient to generate preference.

    Original languageEnglish
    Pages (from-to)20-30
    Number of pages11
    JournalNeuroscience
    Volume250
    DOIs
    Publication statusPublished - Oct 10 2013

    Fingerprint

    Tongue
    Fatty Acids
    Water
    G-Protein-Coupled Receptors
    Knockout Mice
    von Ebner Glands
    Taste Perception
    Glossopharyngeal Nerve
    Taste Buds
    Acids
    Smell
    Dietary Fats
    Linoleic Acid
    Lipase
    Nonesterified Fatty Acids
    Mouth
    Oils
    Triglycerides
    Fats
    Drive

    All Science Journal Classification (ASJC) codes

    • Neuroscience(all)

    Cite this

    Activation of tongue-expressed GPR40 and GPR120 by non caloric agonists is not sufficient to drive preference in mice. / Godinot, N.; Nakano, Keiko; Barcos, M. E.; Pineau, N.; Ledda, M.; Viton, F.; Ninomiya, Y.; le Coutre, J.; Damak, S.

    In: Neuroscience, Vol. 250, 10.10.2013, p. 20-30.

    Research output: Contribution to journalArticle

    Godinot, N, Nakano, K, Barcos, ME, Pineau, N, Ledda, M, Viton, F, Ninomiya, Y, le Coutre, J & Damak, S 2013, 'Activation of tongue-expressed GPR40 and GPR120 by non caloric agonists is not sufficient to drive preference in mice', Neuroscience, vol. 250, pp. 20-30. https://doi.org/10.1016/j.neuroscience.2013.06.043
    Godinot, N. ; Nakano, Keiko ; Barcos, M. E. ; Pineau, N. ; Ledda, M. ; Viton, F. ; Ninomiya, Y. ; le Coutre, J. ; Damak, S. / Activation of tongue-expressed GPR40 and GPR120 by non caloric agonists is not sufficient to drive preference in mice. In: Neuroscience. 2013 ; Vol. 250. pp. 20-30.
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