Activin receptor mRNA is expressed early in Xenopus embryogenesis and the level of the expression affects the body axis formation

Mariko Kondo, Kosuke Tashiro, Gen Fujii, Misaki Asano, Ryutaro Miyoshi, Ryutaro Yamada, Masami Muramatsu, Koichiro Shiokawa

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Activin is a member of the transforming growth factor β (TGF-β) and possesses various activities in cellular control phenomena. During Xenopus embryonic development, activin is thought to act as a natural mesoderm-inducing factor. We isolated here the Xenopus activin receptor cDNA from Xenopus tadpole cDNA library and examined the expression of the Xenopus activin receptor gene during the course of early embryonic development. The Xenopus activin receptor has an 87% homology at the level of deduced amino acid sequence with the mouse activin receptor, and using the cDNA obtained, three bands of mRNA with different lengths were detected in Xenopus embryos throughout early embryogenesis. We synthesized activin receptor mRNA in vitro and tested the effect of the injection of the mRNA into Xenopus fertllized eggs on subsequent development. When the synthetic mRNA was injected into uncleaved fertllized eggs, embryos with reduced trunk structure were formed. However, when the mRNA was injected into the ventral blastomeres at the 16-cell stage, embryos with a secondary body axis were formed. These results indicate the importance of the function of activin receptor in the reguiatory mechanism for body axis formation.

Original languageEnglish
Pages (from-to)684-690
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume181
Issue number2
DOIs
Publication statusPublished - Dec 16 1991
Externally publishedYes

Fingerprint

Activin Receptors
Xenopus
Embryonic Development
Messenger RNA
Activins
Embryonic Structures
Eggs
Complementary DNA
Blastomeres
Transforming Growth Factors
Gene Library
Mesoderm
Genes
Larva
Amino Acid Sequence
Amino Acids
Injections

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Activin receptor mRNA is expressed early in Xenopus embryogenesis and the level of the expression affects the body axis formation. / Kondo, Mariko; Tashiro, Kosuke; Fujii, Gen; Asano, Misaki; Miyoshi, Ryutaro; Yamada, Ryutaro; Muramatsu, Masami; Shiokawa, Koichiro.

In: Biochemical and Biophysical Research Communications, Vol. 181, No. 2, 16.12.1991, p. 684-690.

Research output: Contribution to journalArticle

Kondo, Mariko ; Tashiro, Kosuke ; Fujii, Gen ; Asano, Misaki ; Miyoshi, Ryutaro ; Yamada, Ryutaro ; Muramatsu, Masami ; Shiokawa, Koichiro. / Activin receptor mRNA is expressed early in Xenopus embryogenesis and the level of the expression affects the body axis formation. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 181, No. 2. pp. 684-690.
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