Activin type IB receptor signaling in prostate cancer cells promotes lymph node metastasis in a xenograft model

Masatoshi Nomura, Kimitaka Tanaka, Lixiang Wang, Yutaka Goto, Chizu Mukasa, Kenji Ashida, Ryoichi Takayanagi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Activin, a member of the transforming growth factor-β family, has been known to be a growth and differentiating factor. Despite its pluripotent effects, the roles of activin signaling in prostate cancer pathogenesis are still unclear. In this study, we established several cell lines that express a constitutive active form of activin type IB receptor (ActRIBCA) in human prostate cancer cells, ALVA41 (ALVA-ActRIBCA). There was no apparent change in the proliferation of ALVA-ActRIBCA cells in vitro; however, their migratory ability was significantly enhanced. In a xenograft model, histological analysis revealed that the expression of Snail, a cell-adhesion-suppressing transcription factor, was dramatically increased in ALVA-ActRIBCA tumors, indicating epithelial mesenchymal transition (EMT). Finally, mice bearing ALVA-ActRIBCA cells developed multiple lymph node metastases. In this study, we demonstrated that ActRIBCA signaling can promote cell migration in prostate cancer cells via a network of signaling molecules that work together to trigger the process of EMT, and thereby aid in the aggressiveness and progression of prostate cancers.

Original languageEnglish
Pages (from-to)340-346
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume430
Issue number1
DOIs
Publication statusPublished - Jan 4 2013

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Activin Receptors
Activins
Heterografts
Prostatic Neoplasms
Lymph Nodes
Cells
Neoplasm Metastasis
Epithelial-Mesenchymal Transition
Bearings (structural)
Cell adhesion
Transforming Growth Factors
Tumors
Transcription Factors
Cell Adhesion
Cell Movement
Intercellular Signaling Peptides and Proteins
Molecules
Cell Line
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Activin type IB receptor signaling in prostate cancer cells promotes lymph node metastasis in a xenograft model. / Nomura, Masatoshi; Tanaka, Kimitaka; Wang, Lixiang; Goto, Yutaka; Mukasa, Chizu; Ashida, Kenji; Takayanagi, Ryoichi.

In: Biochemical and Biophysical Research Communications, Vol. 430, No. 1, 04.01.2013, p. 340-346.

Research output: Contribution to journalArticle

Nomura, Masatoshi ; Tanaka, Kimitaka ; Wang, Lixiang ; Goto, Yutaka ; Mukasa, Chizu ; Ashida, Kenji ; Takayanagi, Ryoichi. / Activin type IB receptor signaling in prostate cancer cells promotes lymph node metastasis in a xenograft model. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 430, No. 1. pp. 340-346.
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