Activity and stability of glucose oxidase in molecular films assembled alternately with polyions

Mitsuhiko Onda, Katsuhiko Ariga, Toyoki Kunitake

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Anionic glucose oxidase (GOD) was assembled alternately with polycations poly(ethylenimine) and poly(dimethyldially-ammonium chloride) for molecular film preparation. The enzymatic activity of the films was investigated by sequential redox reaction with glucose, peroxidase and DA67 dye. The enzyme activity of the GOD film was maintained for 14 weeks when stored in buffer and in air at 4 °C. Activity measurement after incubation at elevated temperatures showed that significant deactivation was not observed up to 50 °C, showing that GOD in the film has higher thermostability than native GOD. The pH profile of the GOD activity in the film became broad and shifted towards higher pH than that of native GOD.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume87
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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