Acute glucose overload abolishes Ca2+ oscillation in cultured endothelial cells from bovine aorta: A possible role of superoxide anion

Chiwaka Kimura, Masahiro Oike, Yushi Ito

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Abstract

Effects of acute glucose overload on [Ca2+](i) were investigated in cultured endothelial cells from bovine aorta. Application of 0.1 μmol/L ATP elicited an oscillatory increase in [Ca2+](i) (Ca2+ oscillation) in Krebs solution containing 11.5 mmol/L glucose. The frequency of Ca2+ oscillation induced by ATP increased in a concentration-dependent manner, ranging between 0.03 and 1 μmol/L. When cells were preincubated with 23 mmol/L glucose- containing Krebs solution (high glucose solution) for 3 hours, 0.1 μmol/L ATP failed to induce Ca2+ oscillation but evoked only a phasic followed by sustained increase in [Ca2+](i). Application of a higher concentration of ATP (10 μmol/L) evoked a transient increase in [Ca2+](i) both in control and high glucose-treated cells. However, the falling phase of [Ca2+](i) was prolonged in high glucose-treated cells. Thapsigargin (1 μmol/L), an inhibitor of endoplasmic Ca2+-ATPase, induced a transient followed by a sustained increase in [Ca2+](i) in control cells. Preincubation with high glucose solution increased the rate of rise of the thapsigargin-induced increase in [Ca2+](i) and abolished the sustained increase, suggesting that glucose overload accelerates Ca2+ leak from intracellular store sites and impairs Ca2+ release-activated Ca2+ entry. We found that all of the glucose overload-induced changes in Ca2+ mobilization could be mimicked by xanthine with xanthine oxidase and abolished by superoxide dismutase. These results indicate that acute glucose overload accumulates superoxide anion in bovine aortic endothelial cells, thereby diminishing ATP-induced Ca2+ oscillation through the impairment of Ca2+ homeostasis.

Original languageEnglish
Pages (from-to)677-685
Number of pages9
JournalCirculation research
Volume82
Issue number6
DOIs
Publication statusPublished - Apr 6 1998

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Superoxides
Aorta
Cultured Cells
Endothelial Cells
Glucose
Adenosine Triphosphate
Thapsigargin
Xanthine
Calcium-Transporting ATPases
Xanthine Oxidase
Superoxide Dismutase
Homeostasis

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Acute glucose overload abolishes Ca2+ oscillation in cultured endothelial cells from bovine aorta : A possible role of superoxide anion. / Kimura, Chiwaka; Oike, Masahiro; Ito, Yushi.

In: Circulation research, Vol. 82, No. 6, 06.04.1998, p. 677-685.

Research output: Contribution to journalArticle

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