Ad4BP in the human adrenal cortex and its disorders

Hironobu Sasano, Souichirou Shizawa, Takashi Suzuki, Kazuto Takayama, Takao Fukaya, Ken-Ichirou Morohashi, Hiroshi Nagura

Research output: Contribution to journalArticle

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Abstract

Ad4BP, a zinc finger DNA-binding protein, is a transcription factor that regulates the expression of the steroidogenic P450 genes. We performed immunoblotting and immunohistochemistry of Ad4BP in 34 human adrenal cortex specimens, which included adrenocortical adenomas and carcinomas. Immunoblotting revealed a single band of 53K, corresponding to the mol wt of Ad4BP. The immunohistochemical studies demonstrated that Ad4BP immunoreactivity was present exclusively in the nuclei of nearly all of the adrenocortical parenchymal cells in both the normal and the pathological human adrenal specimens. Ad4BP was immunostained with equal intensity and frequency among the different cell types. Ad4BP immunoreactivity was also observed in areas of marked degenerative changes, such as lipomyelomatous lesions, and in poorly differentiated carcinoma cells. These results suggest a close association of Ad4BP expression with the biological phenotype of adrenocortical parenchymal cells. Ad4BP therefore seems to play important roles in the induction and maintenance of the transcription of all steroidogenic P450 genes in human adrenocortical cells, even after malignant transformation.

Original languageEnglish
Pages (from-to)2378-2380
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume80
Issue number8
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Adrenal Cortex
Genes
DNA-Binding Proteins
Transcription
Zinc
Transcription Factors
Immunoblotting
Cells
Adrenocortical Adenoma
Adrenocortical Carcinoma
Zinc Fingers
Immunohistochemistry
Maintenance
Carcinoma
Phenotype

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Sasano, H., Shizawa, S., Suzuki, T., Takayama, K., Fukaya, T., Morohashi, K-I., & Nagura, H. (1995). Ad4BP in the human adrenal cortex and its disorders. Journal of Clinical Endocrinology and Metabolism, 80(8), 2378-2380. https://doi.org/10.1210/jcem.80.8.7629233

Ad4BP in the human adrenal cortex and its disorders. / Sasano, Hironobu; Shizawa, Souichirou; Suzuki, Takashi; Takayama, Kazuto; Fukaya, Takao; Morohashi, Ken-Ichirou; Nagura, Hiroshi.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 80, No. 8, 01.01.1995, p. 2378-2380.

Research output: Contribution to journalArticle

Sasano, H, Shizawa, S, Suzuki, T, Takayama, K, Fukaya, T, Morohashi, K-I & Nagura, H 1995, 'Ad4BP in the human adrenal cortex and its disorders', Journal of Clinical Endocrinology and Metabolism, vol. 80, no. 8, pp. 2378-2380. https://doi.org/10.1210/jcem.80.8.7629233
Sasano, Hironobu ; Shizawa, Souichirou ; Suzuki, Takashi ; Takayama, Kazuto ; Fukaya, Takao ; Morohashi, Ken-Ichirou ; Nagura, Hiroshi. / Ad4BP in the human adrenal cortex and its disorders. In: Journal of Clinical Endocrinology and Metabolism. 1995 ; Vol. 80, No. 8. pp. 2378-2380.
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