Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily

Shin Ichiro Honda, Ken-Ichirou Morohashi, Masatoshi Nomura, Hiroyuki Takeya, Masato Kitajima, Tsuneo Omura

Research output: Contribution to journalArticle

359 Citations (Scopus)

Abstract

Bovine cytochrome P-450(11β) gene (CYP11B) has six different cis-acting elements, Ad1, Ad2, Ad3, Ad4, Ad5, and Ad6, in the promoter region. The Ad4 site also exists in the promoter regions of other steroidogenic P-450 genes as well as in CYP11B1. An Ad4-binding protein (Ad4BP) which specifically binds to the Ad4 site was purified from bovine adrenal cortex nuclear extract, and its molecular mass was 53 kDa. A complementary DNA encoding Ad4BP was isolated from a bovine adrenal cortex cDNA library. The cDNA clone contained an open reading frame of 1383 base pairs encoding 461 amino acids, whose calculated molecular weight was 51,020. The predicted amino acid sequence of Ad4BP revealed that the protein has a zinc finger domain and a ligand binding/dimerization domain. Ad4BP is a novel member of the steroid hormone receptor superfamily. Comparison of the primary structures of the hormone receptor superfamily showed that Ad4BP was highly homologous to FTZ-F1, which regulates the fushi tarazu gene, and ELP, which is expressed in the murine embryonal carcinoma cells. Transfection of a Ad4BP expression plasmid into CV-1 cells activated the transcription of the CAT reporter gene carrying the Ad4 sequence in the promoter region.

Original languageEnglish
Pages (from-to)7494-7502
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number10
Publication statusPublished - Apr 5 1993

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Steroid hormones
Steroidogenic Factor 1
Steroid Receptors
Genes
Hormones
Genetic Promoter Regions
Adrenal Cortex
Complementary DNA
Steroid 11-beta-Hydroxylase
Embryonal Carcinoma Stem Cells
Amino Acids
Dimerization
Zinc Fingers
Molecular mass
Transcription
Gene Library
Reporter Genes
Base Pairing
Cytochrome P-450 Enzyme System
Open Reading Frames

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Honda, S. I., Morohashi, K-I., Nomura, M., Takeya, H., Kitajima, M., & Omura, T. (1993). Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily. Journal of Biological Chemistry, 268(10), 7494-7502.

Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily. / Honda, Shin Ichiro; Morohashi, Ken-Ichirou; Nomura, Masatoshi; Takeya, Hiroyuki; Kitajima, Masato; Omura, Tsuneo.

In: Journal of Biological Chemistry, Vol. 268, No. 10, 05.04.1993, p. 7494-7502.

Research output: Contribution to journalArticle

Honda, SI, Morohashi, K-I, Nomura, M, Takeya, H, Kitajima, M & Omura, T 1993, 'Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily', Journal of Biological Chemistry, vol. 268, no. 10, pp. 7494-7502.
Honda, Shin Ichiro ; Morohashi, Ken-Ichirou ; Nomura, Masatoshi ; Takeya, Hiroyuki ; Kitajima, Masato ; Omura, Tsuneo. / Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 10. pp. 7494-7502.
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