TY - JOUR
T1 - ADAMTS-13 is produced by glial cells and upregulated after spinal cord injury
AU - Tauchi, Ryoji
AU - Imagama, Shiro
AU - Ohgomori, Tomohiro
AU - Natori, Takamitsu
AU - Shinjo, Ryuichi
AU - Ishiguro, Naoki
AU - Kadomatsu, Kenji
N1 - Funding Information:
We wish to thank M. Iida (Nagoya University) for her excellent technical assistance. This work was supported in part by a Grant-in-Aid for Scientific Research on Innovative Areas (No. 23110002 to K.K.) from MEXT, Japan ; by Grants-in-Aid for Scientific Research (Nos. 18390099 and 20390092 to K.K.) from MEXT, Japan; and by funds from the Global COE Program, MEXT, Japan , to Nagoya University.
PY - 2012/5/23
Y1 - 2012/5/23
N2 - ADAMTS-13, a member of the family of disintegrins and metalloproteinases with thrombospondin motifs, is produced primarily in the liver, particularly by hepatic stellate cells. This metalloproteinase cleaves von Willebrand factor multimers and thereby regulates blood coagulation. Here, we investigated the expression of ADAMTS-13 in the central nervous system. ADAMTS-13 mRNA was expressed in cultured astrocytes and microglia but not in neurons. The protein production of ADAMTS-13 was also detected in these cultured glial cells. Furthermore, we found that the expression of ADAMTS-13 was significantly increased in the rat spinal cord after injury. Supporting the in vivo data, ADAMTS-13 protein was detected in GFAP- and CD11b-positive glial cells in injured spinal cord. Consistent with this, the proteolytic activity of ADAMTS-13 was increased after spinal cord injury. Our data suggest that ADAMTS-13 may have a critical role in the central nervous system, particularly after neuronal injuries.
AB - ADAMTS-13, a member of the family of disintegrins and metalloproteinases with thrombospondin motifs, is produced primarily in the liver, particularly by hepatic stellate cells. This metalloproteinase cleaves von Willebrand factor multimers and thereby regulates blood coagulation. Here, we investigated the expression of ADAMTS-13 in the central nervous system. ADAMTS-13 mRNA was expressed in cultured astrocytes and microglia but not in neurons. The protein production of ADAMTS-13 was also detected in these cultured glial cells. Furthermore, we found that the expression of ADAMTS-13 was significantly increased in the rat spinal cord after injury. Supporting the in vivo data, ADAMTS-13 protein was detected in GFAP- and CD11b-positive glial cells in injured spinal cord. Consistent with this, the proteolytic activity of ADAMTS-13 was increased after spinal cord injury. Our data suggest that ADAMTS-13 may have a critical role in the central nervous system, particularly after neuronal injuries.
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U2 - 10.1016/j.neulet.2012.03.002
DO - 10.1016/j.neulet.2012.03.002
M3 - Article
C2 - 22425718
AN - SCOPUS:84860563928
VL - 517
SP - 1
EP - 6
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -